摘要
目的 探讨TNF -α基因启动子 - 85 0位点C→T多态性与妊娠期高血压疾病 (hypertensivedisordercomplicat ingpregnancy)的相关性。方法 应用聚合酶链反应 -限制性片段长度多态性技术 (polymerasechainreaction -restrictionfrag mentlengthpolymorphism ,PCR -RFLP)检测 10 6例患者和 10 8例健康孕妇的TNF -α基因启动子 - 85 0位点C→T多态性。对两组之间的基因型和等位基因频率进行比较。结果 病例组CT +TT基因型在对照组明显升高 ,18.5 2 %比 7.5 5 % ,χ2 =5 .6 6 ,P =0 .0 17,OR =0 .35 9,95 %可信区间 0 .15 1- 0 .86 5 ,P <0 .0 5 ,T等位基因的发生率在两组间分别为 4 .2 5 %和 2 5 .93% ,χ2 =10 .2 9,P =0 .0 0 1,OR =0 .2 98,95 %可信区间 0 .137- 0 .6 4 8。结论 TNF -α基因启动子 - 85 0位点C→T多态性与妊娠期高血压疾病具有相关性 ,- 85 0位点的突变可能是保护性因素。
Objective: To study whether the development of hypertensive disorder complicating pregnancy is associated with-850C→T mutation at promoter of TNF-α gene. Methods: The -850C→T polymorphism of all the cases were detected by means of PCR-RFLP techniques. Compared the genotype and allele frequencies between patients and controls. Results: A significant different genotype distribution of -850C→T polymorphism was observed between the two groups. Compared with the control group (18.52%), the frequency of CT and TT genotype decreased in the patient group (7.55 %) χ 2=5.66,P=0.017,OR=0.359,95% CI:0.151-0.865,P<0.05. Accordingly, the frequency of allele T in patients is 4.25%, and 25.93% in controls (χ 2=10.29,P=0.001, OR=0.298,95%CI:0.137-0.648). Conclusions: The T allele of -850 at promoter of TNF-α gene may be the protective factor against the development of the hypertensive disorder complicating pregnancy.
出处
《中国优生与遗传杂志》
2005年第1期31-32,35,共3页
Chinese Journal of Birth Health & Heredity
基金
湖北省妇幼保健基金资助 (基金编号 :鄂卫基妇发 [2 0 0 1] 16号 )
关键词
TNF-Α
妊娠期高血压疾病
基因多态性
TNF-α
Hypertensive disorder complicating pregnancy
gene polymorphism
