摘要
目的 :探讨红细胞平均体积 (MCV)、红细胞脆性及血红蛋白 (Hb)电泳联合试验在地中海贫血 (简称地贫 )中的诊断价值。方法 :用日本SysmexKX 2 1自动血细胞分析仪、中山医科大学红细胞脆性一管定量法、美国Helena公司KEP型全自动电泳分析系统 ,分别对本实验室经分子生物学技术确诊的 14 8例地贫及 81例非地贫样品进行MCV、红细胞脆性及Hb电泳测定 ,并对结果作相关统计学分析。结果 :MCV、红细胞脆性、Hb电泳单项试验对地中海贫血诊断的灵敏度及特异度分别为 91.9%、81.1%、83.8%及 79%、91.4 %、90 .1%。MCV与Hb电泳、红细胞脆性与Hb电泳二项试验平行联合的灵敏度及特异度分别为 98.6 %、96 .6 %及 71.6 %、82 .7% ;系列联合的灵敏度及特异度分别为 77%、6 8.2 %及 97.5 %、98.8%。MCV、红细胞脆性、Hb电泳三项试验平行联合的灵敏度、特异度为 10 0 %、6 5 .4 % ;系列联合的灵敏度及特异度为 6 2 .8%、10 0 %。经u检验 ,平行联合试验的灵敏度与各单项试验灵敏度之间、系列联合试验的特异度与各单项试验特异度之间差异有统计学意义 (P <0 .0 5 )。结论 :在地贫的诊断试验中 ,MCV、红细胞脆性、Hb电泳平行联合试验可提高灵敏度 ,系列联合试验可提高特异度 。
Objective:To study the value of the combined test of red cell mean corpuscular volume(MCV)、red cell osmotic fragility(RCOF) test and Hb electrophoresis in the diagnosis of thalassemia.Method:148 samples of thalassemia and 81 samples of non-thalassemia verified by molecular biologic technique were detected by MCV test、RCOF test and Hb electrophoresis.Result:The sensitivity and specificity of single test were as follow: MCV 91.9% and 79%, RCOF 81.1% and 91.4%, Hb electrophoresis 83.8% and 90.1%. The sensitivity and specificity of parallel combined test of MCV and Hb electrophoresis or RCOF and Hb electrophoresis or MCV、RCOF and Hb electrophoresis were 98.6% or 96.6% or 100% and 71.6% or 82.7% or 65.4% respectively. The sensitivity and specificity of serial combined of MCV and Hb electrophoresis or RCOF and Hb electrophoresis or MCV、RCOF and Hb electrophoresis were 77% or 68.2% or 62.8% and 97.5% or 98.8% or 100% respectively. There were significant differences of sensitivity or specificity between single test and the combination of three or two tests.Conclusion:Parallel combined test of MCV、RCOF and Hb electrophoresis increases the sensitivity and serial combined test heightens the specificity in the diagnosis of thalassemia. Combined test is superior to each single test itself.
出处
《临床血液学杂志》
CAS
2005年第1期30-32,共3页
Journal of Clinical Hematology
