摘要
鞘氨醇单胞菌 (Sphingomonassp .)AN1及其原生质体经N+ 离子注入诱变处理 ,蒽的降解率分别提高了 2 9 3%和36 2 % ,总变异率分别为 80 %～ 10 0 %、6 0 %～ 80 % ,耐蒽浓度分别达到 30 0mg/L、4 0 0mg/L ;突变株经 15代遗传稳定性检测 ,有 2株 (AN815 3,AN315 5 )的性状可稳定遗传 ,其降解率分别为 73% ,75 % ,诱变效果显著 .
Sphingomonas sp. AN1 was mutagenized and breeded by N+ implantation, mutants capable of degrading anthrancene was obtained. The research review that the degrading rate increased by 29.3% and 36.2% while the mutagenized rate was 80%～100% and 60%～80%.In addition, the maximum concentration of enduring anthracene reached 300mg/L and 400mg/L. Their heritance stability were examined for fifteen transfers. Among these strains, two strains,AN815-3 and AN315-5,showed predominant character of survival ability and degrading activity. Their rate of degrading anthrancene reached 73% and 75% respectively. Effect of positive mutagenesis is evident.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2005年第1期150-153,共4页
Environmental Science
基金
国家自然科学基金资助项目 (2 0 2 770 2 9)
关键词
离子注入
环境微生物
原生质体
遗传稳定性
ion implantation
environmental microorganism
protoplast
heritance stability
