摘要
目的建立胃浆膜多导联电刺激和胃排空动物模型。方法在12条英国比格犬的胃大弯浆膜层包埋四对心内起搏电极,距幽门40cm空肠近端行一造瘘口。结果①造瘘管收集食糜的方法简单易行,通过其排空量,能了解不同的电刺激和不同的电刺激参数对胃动力的作用。②胃浆膜多导联电极记录的胃体、胃窦慢波电信号清晰、稳定,能准确地记录不同时间和不同实验的胃慢波变化。③单导联和多导长脉冲电刺激均能控制胃慢波。结论胃浆膜多导联电极是研究胃电生理、胃电起搏及胃电起搏对胃排空的影响较理想的方法。英国比格犬是此模型的理想材料。
Objective To set up the model of multiple channels gastric electrical stimulation and gastric emptying. Methods\ Twelve British Beagle dogs were involved in this study .Four pairs of bipolar electrodes were implanted on the stomach serosa along the greater curvature . A jejunal cannula was implanted 20cm beyond the pylorus for the assessment of gastric emptying. Results\ 1. Chyme was easily collected from the jejunal cannula, which represented the effect of various gastric electrical stimulation and various stimulation parameters on gastric motility. 2. Gastric slow waves were recorded clearly and steadily via gastric serosal multiple electrodes. It could record a variety of gastric slow wave in various experiments and various periods. 3. Single_channel and multi_channel electrical stimulation of long pulse via stomach serosal electrodes was able to entrain gastric slow wave. Conclusion\ Multiple channels gastric electrical stimulation is an available method for investigating gastric electrophysiology, gastric pacing and effect of gastric pacing on gastric emptying. British Beagle dogs are ideal materials for this model.
出处
《中国实验动物学报》
CAS
CSCD
2004年第4期223-225,F005,共4页
Acta Laboratorium Animalis Scientia Sinica
基金
与美国德克萨斯州大学医学部胃肠病研究所合作项目。
