摘要
继人类基因组计划完成之后,作为一种新的研究策略,蛋白质组学在生命科学研究中发挥着愈来愈重要的作用。由于生物体系的复杂性和多样性,使得分离效率高、灵敏度高、通量高和动态范围宽的分离分析技术平台的研究和应用已成为蛋白质组学研究的重点和热点之一。着重介绍了近年来应用日益广泛的多维色谱预分离、毛细管液相色谱 质谱联用、毛细管电泳及其与质谱联用等高效分离分析技术在复杂生物体系的蛋白质分析中的最新进展。引用相关文献40篇。
As a new strategy, proteomics is playing more and more important roles in the life sciences after the sequencing of the human genome was completed. Because a biological system is very complex and diverse, the study and application of technological platform for the separation and analysis with high resolution, high sensitivity, high throughput and broad dynamic range have become one of the important and hot spots. The review focused on the new progress of high performance separation and analysis techniques which were applied to the field of proteomics increasingly, such as multidimensional liquid chromatography for pre-fractionation, capillary liquid chromatography coupled with mass spectrometry and capillary electrophoresis interfaced on line with mass spectrometry.
出处
《色谱》
CAS
CSCD
北大核心
2005年第1期26-31,共6页
Chinese Journal of Chromatography
基金
国家自然科学基金(项目号:20275046
20405017)
国家科技攻关计划项目(项目号:2202 BA711A11)
北京市科技计划项目(课题编号:H030230280190)资助.
关键词
蛋白质组学
多维色谱
毛细管液相色谱-质谱
毛细管电泳-质谱
复杂生物体系
proteomics
multidimensional chromatography
capillary liquid chromatography coupled with mass spectrometry
capillary electrophoresis interfaced with mass spectrometry
complex biological system
