摘要
在28℃,pH值6.4-6.8的条件下,用Grace培养基辅以20%的标准胎牛血清培养半消化法接种的家蚕晚期胚。原代培养细胞能够长期存活,观察到了家蚕原代培养过程中分化细胞的有丝分裂过程,发现了原代细胞分裂过程中赤道板的异常和染色体分离的异常现象;培养6个月和10个月的原代细胞分裂百分比分别为0.02±0.01和0.35±0.10,原代细胞异常的有丝分裂细胞百分比逐渐减少,分别为8.0±1.6和3.2±1.0。
The explants used for primary culture are tissue and semi-digestive tissue, which originate from the late embryonic tissue of silkworm (Bombyx mori). The optimal conditions for primary culture are Grace's medium supplemented with moderate amount of antibiotics, 20% fetal bovine serum and incubated at temperature 28℃, pH 6.4-6.8. The whole process of mitosis and abnormal mitosis such as abnormal cell division plate and endonuclear mitosis were found when tissue had been cultured more than 6 months. With the time of culture prolonged, divide ratio increased from 0.02±0.01 to 0.35±0.10, while the percentage of abnormal mitosis decreased from 8.0±1.6 to 3.2±1.0.
出处
《细胞生物学杂志》
CSCD
北大核心
2004年第6期623-626,共4页
Chinese Journal of Cell Biology
基金
国家自然科学基金资助项目(No.30471312)国家高技术研究发展计划资助项目(863计划)(No.2004AA2Z1020)
