摘要
对采用组织块法和酶消化法体外培养小鼠心肌细胞的结果进行了比较,并对体外培养心肌细胞的生理活性进行了初步测定。结果表明,用组织块法分离培养胎、乳鼠心肌可得到自主节律性搏动的心肌细胞;分别用1g/L胶原酶+10g/LBSA,0.5g/L胰酶+1g/L胶原酶,1g/L胰酶分离培养胎、乳鼠心肌,均可得到大量心肌细胞,且细胞活力较好,能自主博动20d以上;用上述2种方法分离培养成年鼠心肌,结果均不理想;在载有自主节律性搏动的小鼠心肌功能性细胞团的培养皿中加入Ca2+和肾上腺素后,细胞团搏动速度加快,搏动力加强;加入K+后则细胞团搏动速度减缓,搏动力减弱,其对钾、钙、肾上腺素的反应与在体内基本一致,表明体外培养的小鼠心肌细胞具有与在体心肌细胞相似的功能。
Two methods of cardiomyocytes culture in mice hearts were designed to evaluate the physiological activities of cultured cardiomyocytes in vitro.The results show that cardiomyocytes of ventricular muscle can be isolated by cultivating tissue pieces.The cultured cardiomyocytes were round-shaped or rod-shaped with spontaneous contractility;with 1 g/L collagenase+10 g/L BSA,0.5 g/L trysin+1 g/L collagenase and 1 g/L trysin,a lot of cardiomiocytes having uitatity can be obtained and can contract spontaneously,but the culture with digestive fluid is not very suitable for adult mice hearts.Supplement with Ca^(2+) and adrenaline in the medium reduced the rate and the power of contract of cardiomyocytes.However the opposite result is obtained after adding K^+.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2005年第1期5-8,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金资助项目(30200137)
国家"863"高技术研究发展计划项目(2002AA216161)
关键词
心肌细胞
体外培养
小鼠
cardiomyocytes
culture in vitro
mice
