摘要
目的 探讨养血清脑颗粒对肾性高血压大鼠 (Renovascularhypertension rat,RH R )的降压作用及对内皮素(Endothelin,ET)、一氧化氮 (Nitric O xide,N O )、降钙素基因相关肽 (Calcitonin gene related peptide,CG RP)等的影响。方法 采用改进的 Goldblatt肾性高血压大鼠模型。造模成功后大鼠共分 6组:模型组、养血清脑颗粒高、中、低 3个剂量组、卡托普利组、牛黄降压丸组,每组 10只,每日灌胃 1次,正常组和假手术组灌服等量蒸馏水。分别在第 1、2 4周的周五、六、日测量大鼠尾动脉收缩压。4周后取血,检测血中 ET、NO 、CGR P 的浓度。 、结果 RH R 模型大鼠血浆中 ET 含量增多,NO 、CG RP 含量降低,养血清脑颗粒各剂量降低血压同时能够降低 ET含量,增加 N O、CG RP 含量,以高剂量组为优 (P <0.05)。结论 养血清脑颗粒能够降低 R H R 血压,并对 ET、NO 、CGRP 等产生一定影响,这可能是降压作用的机制之一。
Objective To evaluate the effects of Yangxue Qingnao Granules (YQG) in decreasing blood pressure in rats with renal hypertension and to investigate its mechanism.Methods Modified Goldblatt renal hypertension rat models were used.The rats were randomized into 6 groups: model group,YQG groups (treated with high-,moderate-and low-dose respectively),Captopril Tablets group and Niuhuang Jiangya Pills group, 10 rats in each group. The model group was given with distilled water and the other groups with corresponding drugs by gavage, once time a day. Blood pressure was detected in 1st, 2nd and 4th week after treatment. Four weeks after treatment plasma levels of endothelin (ET),calcitonin gene related peptide(CGRP) and nitric oxide(NO) were examined by radioimmunoassay.Results Plasma level of ET was increased and those of NO and CGRP were decreased in rats with renal hypertension. YQG could decrease ET level and increase the levels of NO and CGRP, high-dose YQG in particular (P < 0.05).The blood pressure was decreased after one-month treatment of YQG. Conclusion YQG has an effect in decreasing blood pressure of rats with renal hypertension and its machanism may be related to the regulation of plasma levels of ET, CGRP and NO.
出处
《中药新药与临床药理》
CAS
CSCD
2005年第1期20-23,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家十五攻关资助课题(编号2001BA701A07-30)。
关键词
养血清脑颗粒
内皮素
一氧化氮
降钙素基因相关肽
肾性高血压
Yangxueqingnao granules
Endothelin (ET)
Calcitonin gene related peptide(CGRP)
Nitric oxide(NO)
Renal hypertension
