摘要
嗜水气单胞菌β-hemA重组菌PCB的培养上清液经硫酸铵梯度沉淀粗提毒素蛋白质，并在pH2．5的柠檬酸缓冲液里酸化处理以暴露其疏水区，经Mega-10裂解后与QuilA结合制备ISCOMMs，电镜下可观察到30～40nm的多面体的笼格状颗粒结构。经鳗鱼的免疫保护试验证实．ISCOMs组(每尾40～80μg)保护率高达100％，同剂量的溶血素+弗氏佐剂组可达62．5％，未加佐剂的溶血素只有50％。
A method for preparing the immuno-stimulating complexes(ISCOMs)with theβ-hemolysin(β-HemA)and Quil A was described,in which the β-hemolysin of Aeromonas hydrophila was extracted by ammoniumsulphate gradient precipitation and pretreated with an acidic pH(2.5)to reveal hydrophobic regions,and then wasmixed with the Quil A and other reagents.The ISCOMs was a cagelike Structure with a diameter of 30—40 nm bynegative contrast electron microscopy.Several groups of eel were immunized with β-HemA-ISCOMs,β-HemA-CFA and same amount of β-hemolysin without adjuvant,respectively,and followed by challenge with a virlentbacterial strain TPS-30.The protecion rates of that were 100%,62.5%,and 50%,respectively.The resultindicated that the eel immunized with β-HemA-ISCOMs were effectively protected against the challenge.
出处
《福建农业学报》
CAS
2004年第4期238-242,共5页
Fujian Journal of Agricultural Sciences
基金
国家863资助项目(2003AA622050)福建省科技厅资助项目(2000Z077)。
