摘要
目的 :研究医用壳聚糖 (CS)纳米微粒制备的理想条件和影响因素。方法 :采用离子凝胶法 ,以不同浓度 CS溶液与三聚磷酸盐 (TPP)溶液配比反应制备 CS纳米微粒 ,观察了 CS、TPP浓度、CS∶ TPP质量比和反应体系p H值对纳米微粒制备的影响。结果 :反应条件对纳米微粒的制备有重要影响 ,在 CS与 TPP溶液浓度分别为 0 .4～ 2 .5 m g·m l- 1 与 0 .4～ 1.5 m g· ml- 1 时 ,保持质量比在 3∶ 1～ 6∶ 1之间 ,可稳定得到 CS纳米微粒 ;当 p H值为 4 .5～ 6 .0时 CS纳米微粒保持稳定 ,当 p H值 >6 .5则出现沉淀。结论 :离子凝胶法制备 CS纳米微粒简单温和 ,不需使用有机溶剂 ,在上述反应条件下制备 ,可以得到满足生物医学应用要求的
Objective:To study the influence factors and to evaluate perfect conditions for the preparation of chitosan(CS) nanoparticles.Methods:The CS nanoparticles were prepared by ionotropic gelation of CS with tripolyphosphate(TPP) in different concentrations. The influences on the preparation of CS nanoparticles, including the concentrations of CS and TPP solution, the CS:TPP weight ratio and the pH in this reaction system were Observed.Results:There were significant influences on the preparation of CS nanoparticles in different reaction conditions. When the concentrations of CS and TPP solution were in the range of 0.4～2.5mg·ml 1, 0.4～1.5mg·ml 1, respectively, and the weight ratio,between 3∶1~6∶1, CS nanoparticles could be obtained stably.It was also found that the CS nanoparticles were stable in the solution with pH4.5～6.0, but precipitated above pH6.5.Conclusion:CS nanoparticles can be obtained by a mild ionotropic gelation procedure without organic solvents or surfactants.When the concentrations of CS and TPP, the weight ratio and the pH were in the ranges mentioned above, CS nanoparticles which satisfied the demands of application in biomedicine could be acquired.
出处
《南通大学学报（医学版）》
2005年第1期20-22,共3页
Journal of Nantong University(Medical sciences)
基金
江苏省卫生厅 2 0 0 2年重大项目基金 ( H2 0 0 2 15 )
南通医学院创新基金 ( CX0 3 0 1)
