摘要
目的 探讨纳米晶羟基磷灰石胶原复合材料(NHAC)、非纳米晶羟基磷灰石胶原复合材料(HAC)和珊瑚羟基磷灰石(CHA)的体外生物相容性,为组织工程提供理想的细胞基质载体材料。方法 将人骨髓基质干细胞分别与上述三种载体材料体外复合培养,应用倒置显微镜、扫描电镜、四唑盐比色试验(MTT法)及碱性磷酸酶(ALP)活性测定,对材料上复合培养的细胞进行形态学和功能测定。结果 骨髓基质干细胞能在NHAC和CHA两种材料上良好地黏附、增殖和生长。细胞的活性和碱性磷酸酶活性未受到材料的影响;而 HAC不利于人骨髓基质干细胞的黏附、增殖,并使碱性磷酸酶活性降低。结论 NHAC和CHA两种材料具有良好的生物相容性,可作为骨组织工程理想的骨替代材料;HAC不适合做细胞外基质。
Objective To explore the biocompatibility of the nano-hydroxyapatite/collagen(NHAC),hydroxyapatite/collagen(HAC) and coralline-hydroxyapatite(CHA) with human bone marrow stem cell(BMSC) in order to offer the ideal bone material for treating bone defect.Methods The morphologic and fuction of the BMSC were observed by inverted phase contrast microscope,scanning electron microscope,MTT assay and the activity of alkaline phosphatase when coculturing three scaffolds with BMSC in vitro.Results The BMSC can adhere to proliferate and growth normally and perfectly on the surface and holes of NHAC and CHA.The cellular activity and alkaline phosphatase activity were not influenced by NHAC and CHA.HAC not only cannot benefit BMSC to adhere to and growth but also influence badly on the activity of alkaline phosphatase.Conclusion NHAC and CHA have good biocompatibility and can be used as an ideal biomaterial in bone tissue engineering.But HAC is not fit as a scaffold.
出处
《江苏医药》
CAS
CSCD
北大核心
2005年第2期81-83,F002,共4页
Jiangsu Medical Journal
基金
江苏省卫生厅135工程课题(38RC2002038)
苏州大学医学发展基金(EE131031)
