摘要
目的探讨筋膜瓣在促进组织工程骨再血管化及修复山羊长段骨缺损中的作用。方法中国青山羊体内制备单侧胫骨2cm的骨膜与骨缺损,采用空白组、珊瑚羟基磷灰石(CHAP)组、组织工程骨组、筋膜瓣组进行修复。于术后2、4、8、12周采用放射性核素骨显像(ECT)、X线、组织学、生物力学等方法进行对比评价,2、3、6、12、18、30个月采用X线、CT对组织工程骨组进行检测。结果空白组与CHAP组未能修复骨缺损,筋膜瓣组修复骨缺损的效果优于组织工程组,12周筋膜瓣组X线光密度比值、生物力学弯曲应力分别为(4.180±0.192)、(13.937±2.199)N/mm2,组织工程组为(3.480±0.453)、(10.123±1.243)N/mm2,8周ECTROI平均计数筋膜瓣组为26.05±2.61,组织工程组为(16.21±1.46),差异具有统计学意义(P<0.05)。组织工程骨12月以后开始塑形,30个月骨髓腔再通。结论采用筋膜瓣包裹的方法能够显著提高组织工程骨再血管化及修复长段骨缺损的能力。
Objective To study the character and methods of the fascia flap applications during the osteogenesis and revascularization process of tissue engineered bone in Chinese goats tibia defect repair.Methods Two cm left tibia diaphyseal defect was made in 30 Chinese goats.The goats were randomly divided into control group,CHAP group,tissue engineered bone group and fascia flap group (the defect was filled with CHAP+BMSCs + fascia flap).X-ray examination,V-G staining of tissue slice of the implants,the biomechanics of the specimens tested were carried out at week 4,8,12 postoperation.Long-term fOllow-up were performed by X-ray at 6th,12th,18th,30th month postoperation and CT scanning on the month 2,3,6 postoperation.Results In the control group and CHAP group,the defects showed a little trace of bone regeneration.In the fascia flap group and tissue engineered bone group,the defects were repaired.There were significant difference between fascia flap group and tissue engineered bone group ^(P< ^0.05). The optical density index of X-ray film and biomechanics of the specimens tested in fascia flap group 12 weeks postoperation were ^(4.180± ^0.192), ^(13.937± ^2.199) N/mm2,and ^(3.480± ^0.453), ^(10.123± ^1.243) N/mm2 in tissue engineered bone group.The radionuclide bone imaging in fascia flap group 8 weeks postoperation and tissue engineered bone group was ~26.05± ~2.61 and ~16.21± ~1.46 respectively.Tissue engineering bone started modeling at 12th month postoperation and recanalization of bone medullary cavity presented at 30th month postoperation.Conclusion Tissue engineered bone had good ability to repair large segment tibia defect of goat.Fascia flap can accelerate the revascularization and osteanagenesis process of tissue engineered bone.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第3期269-271,i001,共4页
Chinese Journal of Experimental Surgery
基金
国家高技术研究发展"863"计划资助项目(2003AA205010)
国家自然科学基金资助项目(30300367)
