摘要
目的探讨自体骨髓间充质干细胞(MSCs)复合生物活性陶瓷材料β磷酸三钙(TCP)修复羊跖骨节段性缺损的能力。方法将24只成年中国美利奴绵羊随机分成3组,制备单侧跖骨21mm长节段性缺损,缺损分别采用相应方法修复材料+细胞组,缺损区植入βTCP+MSCs复合体(n=10);单纯材料组,缺损区植入单纯βTCP(n=10);空白组,缺损区不作处理(n=4)。术后1、3和6月处死动物并取材,做大体形态学、组织学、Ⅹ线检查。结果术后6月检查结果显示,在材料+细胞组,骨生成能力明显强于单纯材料组;而空白组仅在骨折断面处生成少量松质骨,骨缺损不能修复。结论生物活性陶瓷βTCP与MSCs结合能明显提高骨生成速度,在修复长骨干节段性缺损方面具有潜在的临床应用价值。
Objective To test the ability of autologous bone marrow-derived mesenchymal stem cells (MSCs) to promote repair of critical-size metatarsus gaps upon autologous transplantation on a beta tricalcium phosphate (β-TCP) carrier.Methods Twenty-four adult sheep were randomly assigned to one of the three groups:BMC Group (Bioceramic-MSCs Construct,n=10),BWC group (Bioceramic without cells,n=10) and control group (defect without implant,n=4).A 21 mm metatarsus gap was generated in each sheep and repaired or unrepaired accordingly.Samples were taken 1,3 and 6 months after implantation.Bone-biomaterial interaction was investigated by radiographic,histological,and histomorphometric techniques.Results BMC group conducted a far more extensive bone formation than did BWC group over a 6-month period.But atrophic non-union occurred in the control group,and only a small amount of trabecular bone formed at the cut ends of the cortex of the host bone in this group.Conclusion On large-sized animals the use of autologous MSCs in conjunction with β-TCP-based carriers results in faster bone repair compared to β-TCP alone.Potentially this combination could be used clinically in the treatment of extensive long bone defects.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第3期275-277,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金青年基金资助项目(39900036)
国家"十五"863组织器官工程重大专项课题(2003AA205040)
北京市科技攻关重大项目(H020920050031)
