摘要
目的探讨抗心肌β1 与α1 受体自身抗体是否与糖尿病心肌病发病有关。方法以合成的α 1 受体和 β1 受体多肽片段为抗原 ,应用酶联免疫吸附测定 (ELISA)技术 ,检测196例 ,其中糖尿病心肌病患者46例 ,2型糖尿病52例 ,高血压无靶器管受损患者58例及正常对照组40例血清中抗G蛋白偶联型α 1 和 β1 受体自身抗体。结果糖尿病心肌病组抗 β1 和α 1 受体自身抗体阳性率分别为45.6%和39.1%,明显高于糖尿病无心肌病组的17.3%和17.3% ,高血压组的19%和12.1 %及正常对照组的15%和17.5% ,差异有极显著性(P<0.01)。不同亚组糖尿病心肌病心功能分级抗β1 和α 1 受体自身抗体阳性检出率比较 ,心功能:Ⅱ、Ⅲ级者受体自身抗体阳性率分别55.6 %和44.4% ,而心功能Ⅳ级分别为(1/10)10 %和 (2/10)20%明显低于心功能Ⅱ、Ⅲ级者。结论免疫学机制可能参与糖尿病心肌病病理生理过程。
Objective To explore the role of autoantibodies againstβ 1 receptor andα 1 receptor in the deˉvelopment of diabetic cardiomyopathy.Methods The epitopes of the second extracellular loop ofβ 1 receptor(197～222)andα 1 receptor(192～218)were synthesized and used respectively to screen serum autoantibodies from patients with diabetic cardiomyopathy(DC)(n=46),diabetes mellitus(DM)(n=52),or hypertension(n=58)and from healthy blood donors(n=40,control)by ELISA.Results In diabetic cardiomyopathy,the positive rates of autoantibodies againstβ1-receptor andα 1 -receptor were45.6%and39.1%,respectively.The positive rates were both higher than those of patients with DM(17.3%and17.3%,respectively)and with hypertension(19.0%and12.1%,respectively).They were also higher than those of healthy donors(15%and17.5%,respectively;P<0.01).Conclusions The findings showed higher positive rates of autoantibodies againstβ 1 _receptor andα 1 _receptor in patients with diabetic myocardial disease.These autoantibodies may play imˉportant roles in the pathogenesis of diabetic cardiomyopathy.
出处
《中国微循环》
北大核心
2005年第1期47-50,共4页
Journal of Chinese Microcirculation
