摘要
目的:研究二甲基亚硝胺(DMN)肝纤维化模型MMP- 2,TIMP-1 mRNA表达以及木苏丸的干预作用. 方法:SD大鼠分为正常对照组10只(ip生理盐水), 模型组及治疗组各10只,每周连续3 d,每天ip二甲基亚硝胺10 mg/kg,共3 wk.造模同时模型组每天蒸馏水灌胃,治疗组木苏丸3.125 g/kg蒸馏水溶解后每天灌胃,治疗4wk.心脏取血观察肝功能、血清透明质酸(HA)、超氧化酶歧化物(SOD)及丙二醛(MDA)的改变,以逆转录多聚酶链反应方法观察肝纤维化模型肝组织MMP-2,TIMP-1 mRNA的表达水平及木苏丸的干预作用. 结果:DMN大鼠肝纤维化模型组MMP-2 mRNA为1.38±0.48.TIMP-1 mRNA为0.99±0.14,均高于正常对照组(0.70±0.22 vs 0.63±0.08,P=0.02); 木苏丸治疗组TIMP-1 mRNA为0.71±0.34,低于模型对照组(0.99±0.14,P=0.03);MMP-2 mRNA 为1.29±0.68,与模型对照组无显著差异. 结论:DMN肝纤维化模型肝组织MMP-2,TIMP-1 mRNA表达水平增高,木苏丸能抑制肝组织TIMP- 1 mRNA的表达,从而延缓肝纤维化的形成.
AIM: To investigate the effects of musuwan on the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase 1 (TIMP-1) in rat liver fibrosis induced by dimethylnitrosamine (DMN). METHODS: Male SD rats were randomly divided into three groups: normal control group (n = 10), model group (n = 10) and musuwan group (n = 10). In all the rats, except those in control group, DMN (10 mg/kg) were given by intraperitoneal injection to establish fibrosis model. Rats in the musuwan group were subsequently treated with musuwan (3.125 g/kg, stomach perfusion). Blood from the heart was collected for the examinations of liver function, serum hyaluronic acid (HA), superoxide dismutase (SOD), and malonaldehyde (MDA). MMP-2, and TIMP-1 mRNA were detected by reverse transcription polymerase chain reaction. RESULTS: Compared with those in control group, serum ALP, MDA, and HA were significantly higher, whereas serum levels of Alb and SOD were significantly lower. In musuwan treated rats, serum ALP, MDA, and HA were remarkably reduced, while Alb and SOD were markedly elevated. The levels of MMP-2 and TIMP-1 mRNA in model group were significantly higher than those in control group (0.70±0.22 vs 0.63±0.08, P = 0.02; 1.38 ±0.48 vs0.99±0.14, P = 0.02). Musuwan significantly down-regulated TIMP-1 mRNA (0.71±0.34 vs 0.99±0.14, P = 0.03); however, the level of MMP-2 mRNA showed no significant difference between musuwan group and model group. CONCLUSION: Musuwan can effectively inhibit the expression of TIMP-1, but not MMP-2, in DMN-induced liver fibrosis in rats.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第3期355-357,共3页
World Chinese Journal of Digestology
