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近江牡蛎16S rRNA基因片段序列变异分析 被引量:16

Mitochondrial 16S rRNA Gene Fragment Sequence Analysis in Populations of Crassostrea rivularis
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摘要 采用聚合酶链式反应(PCR)技术扩增了近江牡蛎Crassostrea rivularis (Gould)线粒体DNA16S rRNA基因,获得了大约为500bp的片段.PCR产物纯化后进行序列测定,经Clustal X同源排序,去除引物及部分端部序列,得到了415bp的核苷酸片段.比较并分析了该片段的钦洲湾、长沙湾、镇海湾和珠江口共105个近江牡蛎个体的核苷酸序列多态性,共检测到23个多态性核苷酸突变位点,包括16个转换位点和7个颠换位点,发现了1个核苷酸插入突变位点.4个群体可分为12种单倍型.结果表明:4个群体中广西钦洲湾群体遗传多样性最高,其次为长沙湾、珠江口群体,镇海湾群体最低.
出处 《高技术通讯》 CAS CSCD 北大核心 2005年第2期100-103,共4页 Chinese High Technology Letters
基金 广东省科技厅科技计划,国家高技术研究发展计划(863计划)
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参考文献13

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