摘要
目的 比较咪唑安定、硫喷妥钠、异丙酚拮抗罗哌卡因致大鼠惊厥的作用。方法 SD大鼠50只,随机分为五组,空白对照组(C组):以0.5 ml·min-1速率输注2 ml生理盐水;罗哌卡因组(R组):以0.5 ml·min-1速率静脉输注0.75％罗哌卡因致大鼠惊厥;咪唑安定+罗哌卡因组(MR组);硫喷妥钠+罗哌卡因组(SR组);异丙酚+罗哌卡因组(PR组),每组10只。MR组、SR组、PR组分别于输注罗哌卡因前静脉注射咪唑安定0.23 mg·kg-1、硫喷妥钠2 mg·kg-1、异丙酚1 mg·kg-1,惊厥后即刻处死大鼠,快速取脑,应用反相高效液相色谱荧光法测定脑组织兴奋性氨基酸[天冬氨酸(Asp)、谷氨酸(Glu)]与抑制性氨基酸[甘氨酸(Gly)、γ-氨基丁酸(GABA)]的含量。并计算各组罗哌卡因惊厥剂量。结果 与R组比较,MR、SR、PR组惊厥时罗哌卡因剂量均升高(P<0.05或0.01)。与C组比较,R组、MR组、SR组Asp含量增高(P<0.05或0.01)、PR组差异无显著性(P>0.05),三组Glu含量均增高(P<0.05或0.01)。与R组比较,三组Asp含量均下降(P<0.05或0.01),Glu含量差异无显著性(P>0.05)。与C组比较,R组、MR组、SR组Gly增高(P<0.05或0.01),PR组差异无显著性(P>0.05);GABA含量与Gly变化一致。与R组比较,MR组、PR组Gly与GABA含量降低(P<0.05或0.01),SR组差异无显著性(P>0.05)。结论 三种全?
Objective To assess the anticonvulsant effect of midazolam, thiopental and propofol on the convulsion induced by ropivacaine in rats and the underlying mechanism.Methods Fifty male SD rats, 4-6 months of age, weighing 250-300 g were randomly divided into five groups of 10 animals each : (1) control group (C); (2) ropivacaine group; (R) (3) midazolam-ropivacaine group (MR); (4) thiopental-ropivacaine group (SR) and (5) propofol-ropivacaine group (PR) . In control group normal saline 2 ml was infused i.v. . In ropivacaine group (R) 0.75% ropivacaine was infused i.v. at 0.5 ml·min-1 until convulsion occurred. In midazolam - ropivacaine group (MR) midazolam 0.23 mg·kg-1 was injected i.v. 3 min before 0.75% ropivacaine infusion. In SR and PR groups thiopental 2 mg·kg-1 or propofol 1 mg·kg-1 was injected i.v. 1 min before 0.75% ropivacaine infusion. As soon as convulsion occurred ropivacaine infusion was stopped. The animals were decapitated and their brains were removed and kept in liquid nitrogen for determination of the contents of excitatory ammo-acids (aspartic acid and glutamic acid) and the inhibitory amino-acids [glycine and γ-amino butyric acid (GABA)] by high-performance liquid chromatography . The amount of ropivacaine infused in each group was recorded and calculated.Results The dose of ropivacaine needed to produce convulsion was 4.6±0.5 mg·kg-1 in group R and was significantly increased in the 3 groups pretreated with general anesthetics and was 2.96 times the dose in group R in MR group, 2.84 times in SR group and 1.54 times in PR group. The 4 amino-acid concentrations in the brain were significantly higher in group R than in control group. Compared to group R the Asp, Gly and GABA concentrations were significantly lower in group MR and PR but in group SR only Asp concentration was significantly lower. There was no significant difference in glutamic acid concentration among the4 roopivacaine groups (group 2-5).Conclusion Midazolam, thiopental and propofol can all increase the dose of ropivacaine needed to induce convulsion and midazolam and thiopental are more effective than propofol.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2004年第10期758-760,共3页
Chinese Journal of Anesthesiology
