摘要
目的 :评价Mn DPDP磁共振检查对大鼠脂肪肝的诊断价值。方法 :选取 70只体重为 2 0 0～ 2 5 0gSD健康大鼠 ,随机分为两组 :对照组 10只 ,给以正常饲料和饮用水喂养 ;处理组 60只 ,制成CCL4脂肪肝纤维化模型。从处理组中每周随机抽取第 1～ 13周末各 4～ 5只 ,每只大鼠经尾静脉注射 2 5 μmol/kgMn DPDP ,对肝脏进行FMPSPGR和SET1WI序列扫描 ,扫描延迟时间为 15min、3 0min、1、2、3和 4h。MRI检查后取肝脏大体标本HE染色后进行病理学对照。结果 :大鼠脂肪肝在FMPSPGR序列增强前平扫信号比正常对照组低 ,两组差异有显著性意义 (P <0 .0 5 ) ,不同程度脂肪肝之间差异无显著性意义 (P >0 .0 5 ) ;增强后信号绝对值较对照组低 ,差异有极显著性意义 (P <0 .0 0 1) ,但相互之间差异无显著性意义 (P >0 .0 5 )。大鼠脂肪肝在SET1WI序列增强前平扫信号较对照组高 ,中度和重度脂肪变比对照组和轻度脂肪变信号绝对值高 ,差异有显著性意义 (P <0 .0 5 ) ,二者之间差异无显著性意义 (P >0 .0 5 )。增强后轻度脂肪变信号绝对值较相同时间点对照组低 ;中度脂肪变在增强后 15min信号绝对值较对照组稍低 ,随后各时间点较对照组升高 ;重度脂肪变在增强后各时间点信号绝对值较对照组升高。结论 :大鼠脂肪肝注射Mn
Objective:To evaluate the usefulness of Mn-DPDP enhanced MRI in the diagnosis of fatty liver of rats.Methods:A total of 70 male healthy Sprague Dawley rats weighing 200～250g were divided into two groups.The ten rats as the control group and other 60 rats induced with complex (CCL4,fat,alcohol) were studied with SE T 1WI and FMP SPGR sequences, images were acquired before and after intravenous administration 25umol/kg of Mn-DPDP with identical pulse sequences at 15m,30m,1h,2h,3h,4h; in every week,4～5 rats were randomly chosen and studied with similar MR imaging.Pathological examinations were correlated with MR imaging.Results:On the pre- and postcontrast FMP SPGR T 1WI the mean signal intensity of fatty livers was lower than the normal livers,but there was no significant difference among different grades of fatty livers (P>0.05).On the precontrast SE T 1WI,the mean signal intensity of all fatty livers was higher than that in the controls,especially for the moderate and severe fatty livers.After Mn-DPDP administration,it decreased for the mild at all scanned time;decreased for the moderate at 15min,increased slightly at other delayed time;increased for the severe at all scanned time.Conclusion:Mn-DPDP is a potentially good contrast agent for obtaining prolonged enhancement of the liver,which!has the ability to image fatty liver affecting hepatocelluar metabolism;however,it may not demonstrate differences between grades of fatty liver damage.
出处
《放射学实践》
2004年第12期860-863,共4页
Radiologic Practice
关键词
脂肪肝
磁共振成像
动物
实验
Fatty liver
Magnetic resonance imaging
Animals,laboratory
