摘要
目的 :研究褪黑素对谷氨酸引起的神经细胞Ca2 +内流的抑制作用。方法 :用Ca2 +敏感荧光指示剂Fura -2/AM负载大鼠脑细胞 ,测定神经细胞内游离Ca2 +浓度。结果 :谷氨酸500μmol/L可促进Ca2 +内流 ,显著增加细胞内游离Ca2 +浓度 (P<0. 01) ;应用褪黑素10 -5mol/L后可显著抑制Ca2 +内流 ,降低细胞内游离Ca2 +浓度 (P<0. 01)。结论 :褪黑素可通过抑制谷氨酸引起的Ca2 +内流保护神经细胞。
OBJECTIVE:To study the inhibitory effect of melatonin(MT)on the increased influx of Ca 2+ induced by L-glutamate in cultured rat neurons.METHODS:Fura-2/AM,a Ca 2+ sensitive fluorescent indicator was used to load the brain cells isolated from new-born rats,and the intracellular dissociative Ca 2+ concentration(〔Ca 2+ 〕i)was measured with fluoresˉcence-spectrophotometer.RESULTS:The L-glutamate of concentration500μmol/L could markedly promote the influx of Ca 2+ and intracellular free Ca 2+ concentration(P<0.01),while10 -5 mol/L MT added to L-glutamate could significantly inˉhibit the influx and decrease the concentration.CONCLUSION:MT could protect the cultured rat neurons by inhibiting the influx of Ca 2+ induced by L-glutamate.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第3期174-176,共3页
China Pharmacy
基金
国家自然科学基金资助项目 (39570820)
