摘要
本文以外周血淋巴细胞姐妹染色单体互换(SCE)频率和细胞周期动力学为指标,以较长期(4～13年)生活在砷污染区的黄牛和水牛为对象,对砷在动物体内慢性蓄积所诱发细胞遗传学效应进行了研究。其结果表明,砷污染区黄牛和水牛SCE频率均显著高于对照组,参照有关评价标准,可判为弱阳性,提示在当地污染水平下,黄牛和水牛都诱发了一定程度的细胞遗传学效应。对细胞周期动力学的观察结果表明,砷对黄牛和水牛没有明显的细胞毒性。本试验还报告了正常黄牛和水牛外周血液淋巴细胞SCE的自发频率以及培养72小时各期细胞的正常比率。
Using the frequency of sister chromatid exchange (SCE) of peripheral lymphocytes and cell cycle kinetics as indices, work was done to study the cytogenetic effect on cattle and buffaloes living for a long time in districts subjected to arsenic pollution induced by chronic accumulation of the element in the animal body. The results showed that the SCE frequencies of cattle and buffaloes in the polluted district were significantly higher than those of control animals in clean localities. These were assessed as weak positive reactions according to relerant standards, suggesting that cytogenetic effect to some degree could be induced both in cattle and buffaloes under the conditions of local pollution levels. According to the observed cell cycle kinetics, arsenic showed no obvious cytotoxicity to cattle and buffaloes. The spontaneous frequencies of SCE in peripheral lymphocytes of normal cattle and buffaloes and the normal ratio of cells in different stages at 72 hours of in vitro culture were also reported in this paper.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1993年第2期130-135,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金资助项目
关键词
黄牛
水牛
砷污染
染色体
细胞
Cattle, Buffaloes, Sister chromatid exchange, Cell cycle kinetics, Arsenic pollution
