摘要
利用傅里叶红外光谱研究了 2 0例正常人和 3例鼻咽癌患者手指甲的红外光谱 ,结果表明 :癌指甲样品与正常指甲样品的红外光谱在峰形、峰强度、峰频率等方面均存在明显差异 ;它们的最大区别是 :酰氨Ⅱ带峰形的变化 ,δs(CH3 )峰的消失 ,以及 874 0cm-1附近有无吸收峰。还着重研究了磷酸二酯基团的对称和反对称伸缩振动 ;以及C—O基团伸缩振动峰的变化。并从蛋白质、核酸氢键的角度以及膜脂碳氢链排列及构像变化角度等方面分析了发生变化的原因。磷酸二酯基团的对称伸缩振动峰呈现有规律的变化 ,即癌样品由 10 80 0位移到 10 77 6cm-1,反对称伸缩振动由 12 39 4位移到 12 38 4cm-1,表明指甲核酸分子内磷酸二酯键骨架的结构发生了改变。另外 ,膜脂分子中的CH2 弯曲振动δ(CH2 )的波数也有些改变 ,即癌样品由 14 5 3 1位移到 14 5 3 7cm-1。而且 ,峰的强度也有所增强 ,说明癌患者指甲的膜脂中亚甲基链的无序状态较正常人的大。
Finger nails from twenty normal people and three with carcinoma of nasopharynx were studied by Fourier Transform Infrared (FTIR) technique. The results showed that there are obvious differences between FTIR spectra of them in spectral parameters such as frequency, intensity and band shape etc. The most striking differences in the spectra were observed in the change of amide II, the disappearance of delta(s)(CH3) peak, and at 874.0 cm(-1) whether appeared an absorption peak. The changes involving-the phosphate symmetric stretching upsilon(s), po(2)(-) and asymmetric stretching upsilon(as), po(2)(-) modes, the CH3 and CH2 groups stretching ( upsilon(s), CH2, upsilon(as), CH3) bending (delta(CH3)) modes and the C-O stretching upsilon (C-O) mode were discussed. In addition, the changes of structure of hydrogen-bonding of nucleic,acid and cell proteins and the packing and the conformational structure of the membrane lipids were arialysed. The average wave number of band of upsilon(s) (PO2-) shifted from 1080.0 to 1077.6 cm(-1) and that of upsilon(s) (PO2-) shifted from 1239.4 to 1238:4 cm(-1), which indicated that the degree of hydrogen-bonding formed by oxygen atom of the phosphodiester groups of nucleic acids was weakened. The-Average wave number of band of delta(CH2) of membrane lipids shifted from 1453.1 to 1453.7 cm(-1), and its peak intensity:was slightly enhanced, which suggested that the conformational structure of the methylene chains of membrane lipids is more disordered than in normal nail.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第12期1543-1545,共3页
Spectroscopy and Spectral Analysis
