摘要
从采集于广东的番茄曲叶病病株上分离到病毒分离物G2 ,序列分析结果表明 ,其DNA_A为单链环状 ,全长2 74 4nt,共有 6个ORF ,其中病毒链上编码AV1(CP)、AV2 ,互补链上编码AC1、AC2、AC3和AC4。BLAST结果显示 ,与G2基因组有同源关系的病毒均属双生病毒科菜豆金色花叶病毒属。序列比较结果显示 ,G2与菜豆金色花叶病毒属病毒的DNA_A序列同源率均不超过 83% ,其中同源率最高的是PaLCuCNV_[G10 ](82 8% )。进一步比较发现 ,它们的基因间隔区 (IR)变异最大 (同源率为 30 9%～ 81 8% ) ;CP氨基酸序列的同源率较高 (77 6 %～ 99 2 % ) ,AC4蛋白氨基酸序列的同源率较低 (4 3 5 %～ 78 8% )。系统进化关系分析结果也显示 ,G2与已报道的菜豆金色花叶病毒属病毒的亲缘关系均较远。因此 ,G2可能是双生病毒科菜豆金色花叶病毒属中一个未报道的新种 ,命名为广东番茄曲叶病毒 (TomatoleafcurlGuangdongVirus ,ToLCGDV)
Virus islate G2 was isolated from tomato plants showing leaf curl symptoms in Guangdong. The complete nucleotide sequence of G2 DNA-A was determined to be 2744 nucleotides encoding six potential ORFs, with two (AV1 and AV2) in virus sense and four (AC1, AC2, AC3 and AC4) in complementary sense. G2 DNA-A has a typical characteristics of Begomovirus genome organization. BLAST results showed that G2 DNA-A belonged to the Begomovirus of Geminiviridae. Pairwise comparisons of G2 DNA-A with those of other 41 begomoviruses indicated that G2 DNA-A was most closely related to that of Papaya leaf curl China virus isolate G10 (PaLCuCNV-[G10]) (82.8% sequence identity). The intergenic region (IR) of G2 differed to great extent with other begomoviruses (30.9%～81.8% nucleotide sequence identity); when individual encoded proteins were compared, the CP of G2 had relatively high amino acid sequence identity (77.6%～99.2%)with other begomoviruses whereas the AC4 of G2 had less amino acid sequence identity (43.5%～78.8%). Phylogenetic analysis based on the DNA-A sequences also showed that G2 was less related to the reported begomoviruses. These results revealed that G2 infecting Guangdong tomato might be a previously unreported species of Begomovirus, for which the name Tomato leaf curl Guangdong Virus (ToLCGDV) is proposed.
出处
《微生物学报》
CAS
CSCD
北大核心
2005年第1期48-52,共5页
Acta Microbiologica Sinica
基金
广东省自然科学基金 ( 0 3 1992 )
广东省重点实验室二期建设启动项目 ( 2 0 0 3B60 12 8)~~
关键词
广东番茄曲叶病毒
DNA-A
序列分析
分子特征
Tomato leaf curl Guangdong virus-[G2]
DNA-A
Sequence analysis
Molecular characteristics
