摘要
测定了湍蛙属 6个种共 10个种群 ,以及 4个外群种的线粒体 12S和 16SrRNA基因片段 ,比对后有94 0bp序列 ,发现 35 2个变异位点、 186个简约性位点。运用NJ法、MP法、ML法构建了系统关系树 ,各系统树一致表明内群为一单系群 ,分为两组 :第一组中 ,四川湍蛙两种群先聚合 ,再和棕点湍蛙聚为一支 ;第二组中 ,香港湍蛙和戴云湍蛙聚为一支 ,而香港大屿山离岛湍蛙种群首先与华南湍蛙相聚 ,再与武夷湍蛙构成姐妹支。研究结果表明 :香港地区增加 1种湍蛙分布 ;戴云湍蛙是一有效种 ;四川湍蛙的石棉和洪雅种群间遗传差异达到或超过其他种间的分歧水平。
Mitochondrial gene fragments of 12S and 16S rRNA of six species including 10 populations (Amolops), and 4 outgroup species were sequenced. Aligned sequences showed that there were 940 bp in length with 352 variable sites and 186 parsimony sites. Phylogenetic relationships were analyzed through NJ, MP, and ML methods and got a consistent topological structure, and it indicated that all of the ingroup were clustered together and comprised two groups. In the group Ⅰ, two populations of A. mantzorum were first clustered together as a sister clade to A. loloensis. In the group Ⅱ, A. daiyunensis and A. hongkongensis were clustered together, the Amolops sp. from Lantau Is. of Hong Kong was clustered with A. ricketii together as a sister group to A. wuyiensis. This result indicates that there are at least two species of Amolops in Hong Kong, and that A. daiyunensis is a valid species. The fact that genetic differentiation between the Hongya and Shimian populations of A. mantzorum is even bigger than that between them and A. loloensis strongly implies that there maybe are some unknown species contained in A. mantzorum.
基金
国家自然科学基金资助项目 (3 0 0 0 0 0 18)
国家财政部资助的中国科学院生物科学与技术特别支持费 (STZ -0 1-19)
四川省科技厅重点项目
香港野生生物保护基金会项目
