摘要
本文采用类似系祖建系的方法，对复制型ＨＢＶ转基因小鼠模型的１７、２１、２５三个系列进行了繁育，通过ＰＣＲ和Ｓｏｕｔｈｅｒｎ分子杂交的方法，检测三个系列小鼠尾组织和血清的ＤＮＡ样品，以确定ＨＢＶ基因的整合和复制情况。结果表明，ＨＢＶ基因已稳定地遗传至第五代（Ｆ５），且各世代小鼠血清中都存在ＨＢＶＤＮＡ，此外，整合阳性小鼠的血清中能测出ＨＢＶＤＮＡ的比率很高，Ｆ１代为９４．４４％（１０２／１０８），Ｆ２、Ｆ３代为９５．３１％（６１／６４），故在繁育中可以通过测血清中的ＨＢＶＤＮＡ来确定小鼠是否整合。
n this study, we bred 17, 21, 25 lines of HBV transgenic mice by abreeding methodsim ilar to Sing le-founder Breeding. From the resultsof integration and repl icat ion detccted by Polymerase Chain Reaction(PCR)and Southern blotting, we learnt that HBV gene was transmitted steadilyfrom G0 to F5. Moreover, there was always HBV DNA of integrated inicein the serum. Serum HBV DNA was detectable in a high ratio, F1was 94% (102/103) , F2 F3 was 95.3% (61/64) , respectively. So, we canexactlyscreen transgenic mice from the offspring in the light of serumHBV DNA.
出处
《中国实验动物学报》
CAS
CSCD
1996年第2期71-76,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家"八五"攻关课题
