摘要
采用电视显微镜观察了月见草花粉萌发后花粉管和花粉粒内的细胞器运动，对细胞器运动过程进行了录相，并用计算机对运动图象作了分析。结果表明，月见草花粉内细胞器运动有连续运动和不连续运动两种形式；细胞器运动不依赖于细胞质流动。时快时慢，而且是单向的，没有观察到细胞器的双向运动，细胞松驰素Ｂ能抑制细胞器运动，在萌发的花粉粒内还十分清晰地观察到纤丝束摆动，细胞器沿纤丝运动。
The movement of organelles in the germinated pollen of Oenothera odorata was studied in detail by video microscopy. The image of the organelle movement was processed by com-putergation. The pollen grain of Oenothera odorata is large and easy to germinate in vitro and is suitable for the study of organelle movement . The motion of organelles in the germinated pollen grains and pollen tubes is very vigorous. But the movement of organelles in the pollen tubes is more vigorous than that in the germinating pollen grains. Some of their motion is saltatory. A kind of fibrils was observed in the germinated pollen grains. They are supposed to be made up of actin filaments. Some of the fibrils have one end connected to the plasma membrane and others have both ends linked to the plasma membrane , forming a network. Organelles move along the fibrils continuosely and the speed changes constantly . The speed of movement of organelles is not related to their dimensions. Cytochalasin B can inhibit the movement of organelles. Our results suggest that the movement of organelles is independent of the cytoplasmic streaming in the germinated pollen grains and pollen tubes.
基金
国家自然科学基金资助课题
关键词
花粉
细胞器
月见草
花粉器
花粉粒
Pollen
Organelle movement
Actin fibril
Video microscopy
