摘要
目的:探讨新近发现的毒力相关因子基因群集的左半部分——cag Ⅱ在慢性胃病患者感染幽门螺杆菌(H·pylori)中的检出率及其与H·pylori致病的相关性。方法:采用PCR方法,在107例慢性胃病患者中分离的H·pylori菌株,分别扩增cag Ⅱ中的597pb片段。结果:cag Ⅱ的阳性检出率为69.2 ％,在慢性胃炎、消化性溃疡、胃癌等疾病间的检出无显著差异(P>0.05)。结论:cag Ⅱ在慢性胃病患者不同疾病感染的H·pylori中均有检出。在慢性胃炎、消化性溃疡、胃癌等疾病间的检出率无明显差异,表明cag Ⅱ基因在慢性胃病患者感染H·pylori中的分布与H·pylori毒力的关系并不十分密切,cag Ⅱ的存在意义还有待于进一步研究。
To investigate the distribution of cag Ⅱ in Helicobacter pylori(H·pylori) isolated from Chinese patients, and its relationship to gastroduodenal diseases. Methods Fragment in cag Ⅱ was amplified by polymerase chain reaction(PCR) in 107 H·pylori strains from Chinese patients. Results The amplicom to cag Ⅱ was positive in 74 strains, in which 43 strains come from patients of chronic gastritis and 27 strains from of peptic ulcer and 4 strains from of gastric carcinoma. Conclusion cag Ⅱ distributed in most of Chinese H·pylori strains. No data proved the relation between the existance of cag Ⅱand occurrence of H·pylori related diseases.
出处
《现代消化及介入诊疗》
1999年第4期10-11,共2页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
国家自然科学基金No.39670648)
欧共体委员会科学基金(IC18CT95-0024)
