摘要
我们采用间接酶标葡萄球菌A蛋白(SPA)法对体外培养的人表皮细胞及其异体移植后血型ABH抗原的表达情况进行了观察。发现所有被检培养表皮(n=55)均可查到较弱的与供皮者相一致的血型抗原;培养表皮异体移植后35天内不同时间的活检标本中,可查到供者及受者血型抗原。
The expression of ABH blood group antigens in cultured epidermis and its allograftwas investigated by an immunocytochemical method indirect enzyme conjugated SPA assay.In this study, fifty-five pieces of cultured epidermal sheets, which were cultured in vitrofor one to nine weeks, were examined. The ABH antigens were found in all samples. Incultured epidermis, the blood group antigens were exhibited in a patchy and discontinuousway, unlike the distribution of such antigens in normal skin, which was in a form ofa belt located in the granular and spinous layers of the epidermis. In this study, fourburned patients were grafted with cultured allogeneic epidermal sheets, and the donor anti-gens were found in all biopsies taken from 10 to 35 days after grafting. Ninteen days aftergrafting, the recipient antigens were also detected. The method used in this study to detectABH blood group antigens was proved to have the virtue of high sensitivity and specifi-city. The results observed indicated that the ABH blood group antigens expression of epi-dermis was remarkably reduced during proliferation or after culture in vitro, which mightcontribute to the depression of the antigenic activity of the cultured epidermal allografts.
