摘要
取池塘内活蝌蚪制成提取液(T-871),以1∶50(V/V)浓度与RPMI 1640培养基混合制成T-871培养液,培养HeLa细胞。结果证明:T-871可引起HeLa细胞膜Na^+-K^+-三磷酸腺苷酶活性下降,ConA受体增多,乳酸脱氢酶释放减少,G_2期和M期细胞堆积,而对细胞DNA和RNA影响不明显。由此推测,T-871诱导HeLa细胞分化的机理可能是细胞膜结构发生改变,从而降低了细胞恶性。
The tadpole extract without large molecular protein(T-871) was prepared from dry tadpoles. This extract and RPMI1640 medium were mixed in the ratio of 1:50(V/V) to form T-871 medium. HeLa cells were cultured in the T-871 medium in order to study the possible mechanism of HeLa cell differentiation induced by tadpole extract. We found that there was a decreased acitivity of LDH which may be released through HeLa cell membrane in the 2 day's cultured T-871 medium. After 3 day's culture in the T-871 medium we found that on the HeLa cell membranes, Na-K-ATPase activity reduced and the content of Con A receptors increased. When these cells were analyzed by flow cytometry(FACS-420), the results indicated that HeLa cells accumulated in G_2 and M phases of the cell cycle and the influence on DNA and RNA content of the HeLa cells was insignificant. HeLa cells cultured by RPMI 1640 medium or T-871 medium for 3 days were inoculated into the dorsal hypodermis of 12 nude mice, respectively. Then after 24 hours this extract was injected to each nude mouse at dose of 0.3 ml each day. Our study showed that T-871 could result in decrease of tumor formation such as delay of tumor nodule appearance and reduce of tumor weight. This study suggested that the changes of the cell membrane may be caused by the tadpole extract. It may also reduce malignancy of the HeLa cells.
出处
《解剖学报》
CAS
CSCD
北大核心
1993年第1期68-72,T009,共6页
Acta Anatomica Sinica
关键词
蝌蚪
HELA细胞
酶
细胞化学
Tadpole extract
HeLa cell
Differentiation
Cell culture
Enzyme cytochemistry
