摘要
本实验利用大鼠甩尾测痛结合脊髓蛛网膜下腔注药的方法，观察了脊髓水平内源性阿片样物质（ＯＬＳ）对５一羟色胺（５—ＨＴ）４痛作用的影响。实验结果显示；（１）脊髓蛛网膜下腔注射６０ｎｍｏｌ的５—ＨＴ可产生明显的镇痛作用，并可为同一途径注射阿片受体阻断剂纳络酮所完全阻断；（２）同一剂量的纳络酮也能阻断鞘内注射６０ｎｍｏｌ吗啡所引起的镇痛作用；（３）单独使用１２０ｎｍｏｌ纳络酮对痛阈无明显影响。以上结果表明．在脊髓水平５—ＨＬ的镇痛作用是通过ＯＬＳ介导的。
The effect of naloxone on the analgesia produced by intrathecally administered (i. t )serotonin (5 ——HT) or morphine was observed and compared in rats using tail ——- flick test. The results showed that: (l )The analgesia produced by 5 —— HT (60nmol, i, t. )could be blocked by naloxone (120nmol,i. t. ) ； (2 )The analgesia produced by morphine(60nmol,i. t )could also be blocked bythe same dosage of naloxone; (3) 120nmol naloxone administration alone had no effect on the tail—— flick latency;This suggests that 5 —— HT——induced analgesia is mediated by endogenous opiate like substances(OLS) at the spinal level.
出处
《长治医学院学报》
1994年第4期291-295,共5页
Journal of Changzhi Medical College
关键词
吗啡
纳络酮
血清素
阿片样物质
: tail —— flick latency ； serotonin ； morphine ； naloxone ； intrathecal injection
