摘要
角叉菜胶（Ｃａｒｒａｇｅｅｎｉｎ，Ｃａｒ）致大鼠胸膜炎后８ｈ，胸膜腔渗出液中中性白细胞游离钙浓度（Ｎｅｕ－［Ｃａ（２＋）］ｉ）升高，中性白细胞钙调素活性（Ｎｅｕ－ＣａＭＡ）增强；粉防己碱（Ｔｅｔｒａｎｄｒｉｎｅ，Ｔｅｔ）（１０～８０ｍｇ·ｋｇ（－１））于致炎前３０ｍｉｎ和致炎后４ｈ给大鼠灌胃可明显减少炎症胸膜腔渗出液量、蛋白渗出量和白细胞游出数，同时能降低Ｎｅｕ－［Ｃａ（２＋）］ｉ和Ｎｅｕ－ＣａＭＡ；在体外，Ｃａ（２＋）（３２μｍｏｌ·Ｌ（－１））能不同程度的对抗Ｔｅｔ（２０～１６０μｍｏｌ·Ｌ（－１））抑制Ｎｅｕ－ＣａＭＡ的作用。提示：Ｔｅｔ具有良好的抗炎作用，其机理与抑制Ｃａ（２＋）－ＣａＭ系统有关，Ｔｅｔ抑制Ｎｅｕ－ＣａＭＡ可能与其降低Ｎｅｕ－［Ｃａ（２＋）］ｉ有关。
There was an increase of cytosolic free calcium concentration [Neu-Ca(2+)]i and calmodulin activity(Neu-CaMA)of the neutrophils in the pleural exudate at 8 h after carrageenin-induced pleuritis in rats. When tetrandrine(Tet) was intragastrically administered in the dosages of 10, 20, 40 and 80 mg.kg(-1) to rats at 30 min prior to and 4 h after the infliction of pleuritis, the amount, protein content and neutrophil count of the pleural exudate could significantly reduced,[Neu-Ca(2+)]i was decreased and Neu-CaMA inhibited. In addition, it was found that calcium was able to reverse the inhibition of tetrandrine on Neu-CaMA in vitro.On the basis of these findings, it is believed that the excellent anti-inflammatory effect of Tet may be related to its antagonism to calcium-calmondulin system and the inhibition of Tet on Neu-CaMA is dependent upon the decrease of [Neu-Ca(2+)]i level.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1994年第3期157-160,共4页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
胸膜炎
药物疗法
角叉菜
粉防己碱
pleurisy / DT
tetrandrine
carrageenan
inflammation
neutrophils
calcium
calmodulin
Tetrandrine
