摘要
本文研究了用乙二胺作为硅胶柱改善剂的高效液相色谱法,对操作条件进行了探讨.采用示差折光检测器检测,含0.01％乙二胺的已腈-水(72:28,V/V)作流动相,在硅胶柱上成功地分离了果糖、葡萄糖、蔗糖、麦芽糖、乳糖和棉三糖。定量地分析了几种鲜桃汁中糖的含量.
The method was described for the separation of frutose, glucose, sucrose, maltose , lactose and raffinose by high-performance liquid chromatography on plain silica column with acetonitrile-water (72 : 28 ,V/V) containing ethylenediamine modifier (0. 01%, V/V) as the mobile phase. The eluates were quantitatively determined by RI detection with peak height method. The method has been applied to the determination of the concentration of sugars in fresh peach juice samples with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1994年第9期869-872,共4页
Chinese Journal of Analytical Chemistry
关键词
乙二胺
硅胶柱改善剂
糖
液相色谱
Sugars, Ethylenediamine, High-performance liquid chromatography, Fresh peach juice.
