摘要
用三种方法测定血清免疫球蛋白（Ｉｇ）含量，并进行了彼此间差异的显著性和精密度的比较，认为三种方法测定血清Ｉｇ的含量无显著性差异（Ｐ＞０．０５），彼此间相关性较好（ｒ＝０．９７７）。而用免疫比浊法（ＩＴＭ）测定血清Ｉｇ的精密度远高于单向免疫扩散法（ＲＩＤ）和火箭免疫电泳法（ＥＩＡ）。用免疫比浊法测定ＩｇＧ、ＩｇＡ、ＩｇＭ的正常参考值（Ｘ±ｓ）分别为１２．１３±１．５１ｇ／Ｌ、２．０４±０．５５ｇ／Ｌ、１．１４±０．２７ｇ／Ｌ。慢性活动性肝炎ＩｇＧ、ＩｇＡ水平１９．８８±４．９６ｇ／Ｌ３．３３±０．６８／Ｌ与正常人比较差异有高度显著性（Ｐ＜０．０１）；ＩｇＭ水平１．２６±０．２４ｇ／Ｌ与正常人比较无显著性差异（Ｐ＞０．０５）．同时用免疫比浊法测定ＩｇＧ、ＩｇＡ、ＩｇＭ有较好的回收率。
Three methods were used to detect serum immnoglobin and their precision and statistical significance compared. We thought there was not significant difference between these methods(P>0. 05)and there was a high correlation(r=0.97). Precition of immunoturbidimetry(ITM) is far higher than that of radical immunodiffusion(RID) and rocket eletroimmunoassay (EIA). Normal reference values of ITM detecting IgG, IgA, IgM, were 12.13±1.51 g/L2.04±0.55 g/L1. 14±0. 27 g/L. Respectively IgG, IgA, level values of chronic active hepatitis were 19. 88±4. 96 g/L, and 3. 33±0. 68 g/L, and compared with the normal there was significant difference(P<0.01). IgM Value was 1.26±0.24 g/L, compared with normal there being not significant difference(P>0. 05) in comparison with the normal.Moreover, there were good retrieval.
关键词
免疫测定
免疫球蛋白类
血液
化学
immunoassay/MT
Immunoglobulins/BL
chemistry
clinical/MT
compartive study
