摘要
本文应用核酸分子杂交技术对喉癌组织中Ｃ－ｍｙｃ癌基因扩增进行了研究，结果发现：①喉癌组织中具有Ｃ－ｍｙｃ癌基因扩增，其平均扩增倍数为正常喉组织的２．３倍。②Ｃ－ｍｙｃ癌基因扩增水平与喉癌临床分期有关。表现为Ｎ期肿瘤Ｃ－ｍｙｃ基因扩增明显高于Ｉ期和Ⅱ期，Ⅲ期又明显高于１期。本研究结果表明：在喉癌组织中Ｃ－ｍｙｃ基因以扩增形式被激活，激活后的癌基因对喉癌的发生、发展可能起着重要的作用；Ｃ－ｍｙｃ基因扩增与喉癌发展阶段相关，其扩增程度随着肿瘤的病期增高而增高，揭示Ｃ－ｍｙｃ癌基因扩增水平有可能作为肿瘤病情进展和恶性度的指标。本实验从基因水平上探讨了喉癌组织中Ｃ－ｍｙｃ癌基因的激活方式，为今后进一步研究喉癌发生发展机理以及对肿瘤进行基因诊断、治疗提供了实验依据．
C-myc amplification in 22 squamouscell carcinomas and 10 normal tissues(control)from the larynx was assessed by dot hybridization tech-nique。Significant amplification of C-myc oncogenewas found in malignant tumours as compared with thenormal tissues。 The extent of amplification also corre-lated with advanced tumour stages。 There was a sig-nificant increase in C-myc amplification in TNM stages Ⅲ and Ⅳ when compared with the stages Ⅰ and Ⅱ。These data suggested that C-myc oncogene might beactivated by gene amplification and it might have a keyrole in determining the aggressiveness of laryngeal car-cinoma
出处
《华西医科大学学报》
CSCD
1994年第1期41-44,共4页
Journal of West China University of Medical Sciences
