摘要
以０．１ｍｏｌ／ＬＮａＯＨ为溶媒，１８种组成蛋白氨基酸在２８０～３１０ｎｍ波长，仅色氨酸和酪氨酸有吸收峰。利用酪氨酸在两特定波长处有等吸光度值来消除复合氨基酸中酪氨酸对色氨酸的干扰，导出两特定波长处氨基酸吸光度差值△Ａ与色氨酸浓度Ｇ＿（Ｔｒｐ）的回归方程：△Ａ＝－０．００３４９７３＋３．８０４８Ｃ＿（Ｔｒｐ），相关系数ｒ＝０．９９９９，测得平均回收率为９９．８％，变异系数ＣＶ＝１．００％
There were only absorbing peaks of tryptophan and tyrosine in 2 80～310 nm wavelengthsin 18 amino acids with 0.1 mol/LNaOH as the solvent. The tryptophan was measured in twowavelengths of equal tyrosine ABS(λ_1=300 nm,λ_2=284.5 nm) with eliminating interferenceof tyrosine. Regression equation :△A=-0. 003 497 3 + 3. 804 8 C_(Trp). Relative coefficient e-quated 0. 999 9(r=0.999 9).Average rate of recovery was 99.8%。Variation coefficient was1.00%(CV=1.00%)。
出处
《江西农业大学学报》
CAS
CSCD
1994年第4期404-407,共4页
Acta Agriculturae Universitatis Jiangxiensis
