摘要
用硝基苯预处理前后的溶菌酶水溶液激光喇曼散射光谱，经过二阶导数处理，证明在反映蛋白质分子主链构象的酰胺Ⅱ与酰胺Ⅰ区域内，各种特征训线在硝基苯预处理前后位置基本未变，不影响对蛋白质二级结构的分析．
The Raman spectra of native Lysozyme in aqueous solution and the Lysozyme extracted by Nitrobenzene have been obtained. Using the second derivative method for Raman signals of amide 1 and amide Ⅲ regions which reflect main chain con formation of protein molecule, it has been proved that the various characteristic bands of protein main conformation appeared almost all in original sites after Nitrobenzene pretreatment, and there is no fluence on the secondary structure analysis of the protein.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
1994年第2期54-58,共5页
Journal of Nanjing Normal University(Natural Science Edition)
基金
江苏省教委自然科学基金
