摘要
在等位酶分析中，对酶谱的正确解释是获取遗传学资料的基础，酶谱作为酶基因的表现型，是由该种酶蛋白质的四级结构情况（亚基的数目）、在亚细胞分室中的分布（位点的数目）以及所分析样品的倍性和基因型的情况所决定的。术语“酶型”被建议用来记录和描述各种情况下的酶谱的不同。在进行生物多样性和分子系统学研究中，如果直接把酶谱上带的数目的多少作为遗传多样性大小的指标，或把带的多少及迁移率的大小作为数量性状进行聚类和分枝分析将会得出非常错误的结论。
The correct interpretation on the zymogram is the key to acquire the genetic data, in allozyme analysis. As aphenotype, the banding pattern in a zymogram is based on the quaternary structure of the enzyme protein(the number of subunits),the distribution in the subcellular compartments of the enzyme (the number ofloci), and the ploidy and the genotype of the sample examined. A term of 'Zymotype' is suggested to usefor iterpreting the panding patterns. It will lead to a completely wrong conclusion, if the numbers of thebands or the differences of migration rate of the bands are directly used as a measure of genetic diversity or asa numerical characters to calculate the similarity and to make a cluster analysis for a group of samples.
出处
《生物多样性》
CAS
CSCD
1994年第3期149-156,共8页
Biodiversity Science
基金
国家自然科学基金
中国科学院生物分类区系特别支持费资助
关键词
等位酶
分析
同功酶
遗传多样性
allozyme analysis, isozyme, zymotype, genetic diversity, molecular systematics
