摘要
从树舌子实体中分离纯化出另两个级分Ｆｂ、Ｆｃ，经超速离心，电泳，ＳｅｐｈａｒｏｓｅＣＬ－４Ｂ柱层析等确定其均为质点、极性均一级分。经ＧＣ、ＩＯ＿４～－氧化，Ｓｍｉｔｈ降解，部分酸水解，甲基化及其产物ＧＣ，ＧＣ－ｍｓ分析，ＩＲ等分析确定Ｆｂ是由１→３甘露糖基构成主链，分枝点率及分枝率均较大；Ｆｃ由１→６和１→４甘露糖基构成主链，分枝点率及分枝率较少。
Water soluble crude polysaccharide from the fruit bodies of Ganoderma Applana tum were fractionated into two fractions Fb ,Fc. They give single, symmetrical peaks on ultracentifugation,on Sepharose CL-4B and electrophoresis. Periodate oxidation, Smith degradation, partial hydrolysis, methylation analysis, IR,13C-NMR etc were empl0oyed for the structure analysis of fractions. These structure analysis indicated that Fb is a hetero polysaccharide containing a main chain of 1-3 manophyranosyl residues substituted at 0-6, and Fc containing a main chain of 1-6, 1-4 manophyranosyl residues substituted at 0-3. There are more chains in Fb than in Fc on molocule basis.
关键词
树舌
甘路聚糖
多糖
Ganoderma Applanatum
Mannan
