摘要
用气相色谱法测定鳀鱼鱼油中ＥＰＡ、ＤＨＡ的含量，鱼油ＰＵＦＡ用０．５ｍｏｌ／ＬＮａＯＨ－甲醇液皂化，ＢＦ＿３－甲醇酯化成脂肪酸甲酯，进行气相色谱测定。内标物用ＴＳＡ－Ｍ。模拟试验５份平均回收率ＥＰＡ－Ｍ为８９．８６％，ＤＨＡ－Ｍ为８９．７８％。变异系数ＥＰＡ－Ｍ为０．８０％，ＤＨＡ－Ｍ为０．６４％。表明本法简便、快速、重现性好。
In this paper the method of gas chromatographic (GC) analysis for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the anchovy oil was reported. The polyunsaturated fatty acids(PUFA)in the fish oil were saponified with 0. 5 mol/L NaOH-CH3OH, and esterified with BF3-CH3OH for GC analysis. The quantity was determined by internal standard method with TSA-M as a reference. In five determinations, the average rate of reclamation for EPA-M and DHA-M was 89. 86%, 89. 78% respectively. The variation coefficient for EPA-M was 0. 80%, and for DHA-M was 0. 64%. The proposed method is simple, rapid and reproducible.
