摘要
本文研究了淡水鱼类精子在低温冷冻保存时，其渗透压在抗冻剂ＤＭＳＯ作用下的变化规律。冷冻前精子的渗透压在ＤＭＳＯ作用下最高可达１５３８ｍＯｓｍ／Ｌ与４．５％ＮａＣｌ溶液相等渗，是自然条件下的５倍。解冻后最高仍保持在１３６７ｍＯｓｍ／Ｌ与４．０％ＮａＣｌ溶液相等渗，是自然条件下的４．５倍。渗透压变化（Ｙ）与二甲亚矾浓度（Ｘ）的关系式为Ｙ＝０．１４８ｘ＋１．２４５，ｒ＝０．８９８（冻前）和Ｙ＝０．１３６ｘ＋０．６４１．ｒ＝０．９６８（冻后）。当冷冻前精子渗透压为１０２５－１３６７ｍＯｓｍ／Ｌ时进行冷冻保存，解冻后精子成活率最高。
The mechanism of cryoprotection of fish spewn by dimethylsulfoxide(DMSO) lie is itsfast penetration into the sperm and increase of the osmotic pressure of the sperm, Results ofthis study showed that the osmotic pressure of the spenn increased in direct proportion to thetreatment time and concentration of DMSO,Before cryopreservation, when the spem wastreated with 14 % DMSO for 5-7h,its osmotic pressure rose to a peak level of 1538mOsm / L,which was five times higher the level under natural conditions.After thawing,theosmotic pressure of the sperm was still maintained at 1025 mOsm / L,or three times the levelunder natural conditions.When the DM SO concentration increased to 22% prior tocryopreservation, sperm osmotic pressure rose to 1538 mOsm / L immediately, and wasmaintained at 1367 mOsm / L after thawing. After thawing,the spenn osmotic pressure was 171-513 mOsm/ L lower than beforethawing.The survival rate after thawing was highest when the osmotic pressure of the spennprior to cryopreservation was 1025-1367 mOsm / L. As the spenn osmotic pressure afterthawing was 3-4.5 times higher than that under natural conditions,it was necessary to acti-vate the speml with 1.0-2.0% NaCl to achieve higher survival rate.The relationship between sperm osmotic pressure(Y)and DMSO concentration(X)was:Y=0.148X+1245,r=0.898(Osmotic pressure before thawing) and Y=0.136X+0.641,r=0.968(Osmptic pressure after thawing)
出处
《水生生物学报》
CAS
CSCD
北大核心
1994年第4期297-302,共6页
Acta Hydrobiologica Sinica
基金
国家"七.五"科技攻关
关键词
二甲亚砜
淡水鱼
精子
渗透压
成活率
Dimethylsulfoxide(DMSO),Freshwater fish spenn,Osmotic pressure,Sur-vival rate
