摘要
利用 8 0个 1 0bp随机引物对亚洲百合‘pollyanna’及辐射种球诱导出的 2 0个表现型雄性不育突变体进行了RAPD分析。其中有 31个引物对所有材料都能扩增出理想的带型 ,有 4个随机引物扩增出的带型显示其中的‘P1G0 3’、‘P2G0 4’等 9个突变体与‘pollyanna’基因组 (可育系 )之间具有稳定的多态性差异。它们表现出与‘pollyanna’差异的多态性位点有 7～ 1 8个 ,表明它们为‘pollyanna’的基因型突变体。
RAPD analysis of 80 random 10-mer primers on Lilium Asiatic hybids 'pollyanna' and its 20 phenotype male sterility mutants induced by irradiation was carried out. Of the tested primers, 31 primers could produced ideal amplification bands on all materials, 4 primers generated stable different polymorphic bands among 9 mutants and ‘pollyanna’. Different polymorphic bands of 7～18 were found among 9 mutants and ‘pollyanna’. It was showed that 9 mutants were phenotype male sterility mutant of ‘pollyanna'.=
出处
《核农学报》
CAS
CSCD
北大核心
2005年第1期29-32,共4页
Journal of Nuclear Agricultural Sciences
基金
北京市自然科学基金资助项目 (3 9770 45 7)
北京市科技新星项目 (2 0 0 3B13 )
