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Carbendazim resistance and calculation effective concentration of carbendazim for Trichoderma harzianum

Carbendazim resistance and calculation effective concentration of carbendazim for Trichoderma harzianum
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摘要 There is a method for investigating the transformation of resistance gene of carbendazim into Trichoderma harzianum. In order to introduce the resistance to benzimidazole fungicide into bio-control microorganism Trichoderma harzianum was transformed with the resistance gene. In this study, we investigate resistance level and calculate EC 50 (effective concentration of carbendazim that can survive 50% of Trichoderma harzianum in that concentration) and stability of the resistance for the transformant isolate of Trichoderma harzianum. Results show the transformants can growth on the medium containing more than 1 000 μg/ml carbendazim and the resistance is stabled after 10 times transfer on non-selective medium and have EC 50 average about, 1 200 μg/ml. There is a method for investigating the transformation of resistance gene of carbendazim into Trichoderma harzianum. In order to introduce the resistance to benzimidazole fungicide into bio-control microorganism Trichoderma harzianum was transformed with the resistance gene. In this study, we investigate resistance level and calculate EC 50 (effective concentration of carbendazim that can survive 50% of Trichoderma harzianum in that concentration) and stability of the resistance for the transformant isolate of Trichoderma harzianum. Results show the transformants can growth on the medium containing more than 1 000 μg/ml carbendazim and the resistance is stabled after 10 times transfer on non-selective medium and have EC 50 average about, 1 200 μg/ml.
作者 Elham Siassi YANGQian
机构地区 Dept. of Biotechnology
出处 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2005年第1期21-24,共4页 哈尔滨工业大学学报（英文版）
关键词 fungicide resistance CARBENDAZIM trichoderma harzianum EC 50 杀虫剂 真菌 抵抗能力 木霉属 毒性 农药
分类号 TQ453 [化学工程—农药化工] X592 [环境科学与工程—环境工程]
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参考文献11

  • 1YANG Qian ZHAO XiaoyanCollege of Forestry, Northeast Forestry University, Harbin 150040, China,Harbin Veterinary Institute, Harbin 150040, China.Method of transforming resistance gene to carbendazim into Trichoderma sp.[J].Chinese Science Bulletin,1999,44(1):65-68. 被引量：1
  • 2CHEN Zhangliang.Application of plant genetic engineering in Agriculture[].High Technique News Letter.1992
  • 3NEFF N F,THOMAS J H,GRISAFI P,et al.Isolation ofthe beta-tubulin gene from yeast and demonstration of its essential function in vivo[].Cell.1983
  • 4MARK S G,RAYMOND J S L,SRIRAMA B,et al.Pathogenicity and growth of metarhizium anisopliae stably transformed to benomyl resistance[].Current Genetics.1990
  • 5LUO K,REN X G,ZHOU B W.Study on the parasitie fungi on sclerotia of Sclerotinia sclerotiorum in rape[].Oil Crops ofChina.1987
  • 6SONG Jinzhu,YANG Qian,WEI Zhigang,et al.A study on biocontrol mechanism ofTrichoderma spp[].With Emphases on Antagonistic Capability Against Sclerotinia Sclerotiorum and Tolerance to Carbendazim.2000
  • 7ELAD Y,CHET I,BOYLE P,et al.Parasitism of Trichoderma spp: On Rhizoctonia solani and Sclerotium rolfsii Scanning electron microscopy and fluorescence microscopy[].Phytopathology.1983
  • 8BENHAMOU N,CHET I.Hyphal interaction between Trichoderma harzianum and Rhizoctonia solani:Ultrastructure and gold chemistry of the mycoparasitic process[].Phytopathology.1993
  • 9YANGQian.ResistanceofPlantPathogentoFungicides[]..1995
  • 10HUANGDafang,BHAIRIS,STAPLESRC. BiologicalEngineeringPrograss . 1990

二级参考文献11

  • 1Vesa V. Joutsjoki,Tuula K. Torkkeli,K. M. Helena Nevalainen.Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei[J]. Current Genetics . 1993 (3)
  • 2Mark S. Goettel,Raymond J. S. Leger,Srirama Bhairi,M. Katherine Jung,Berl R. Oakley,Donald W. Roberts,Richard C. Staples.Pathogenicity and growth of Metarhizium anisopliae stably transformed to benomyl resistance[J]. Current Genetics . 1990 (2)
  • 3MarkSG,RaymandJSL,SriramaB,etal.PathogenicityandgrowthofMetarhiziumanisopliaestablytransformedtobenomylresistance犤J犦Currentgenetic,1990.
  • 4H Koenraadt,SC Somerville,AL Jones.Characterization of mutations in the beta-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi. Phytopathology . 1992
  • 5Sheir-Neiss,C,Lai,MH,Morris,NR.Identification of a gene for B-tubulin in Aspergillus nidulans. Cell . 1978
  • 6NF Neff,JH Thomas,P Grisafi,D Botstein.Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo. Cell . 1983
  • 7Yang Qian.Resistance of Plant Pathogen to Fungicides. . 1995
  • 8Joutsjoki,VV,Torkkeli,TK,Nevalainen,KMH.Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei. Current Genetics . 1993
  • 9Huang, D. F,Bhairi, S,Staples, R. C.Study on transforming Botrytis squamosa with the plasmid (pDH 25) with theresistance gene to Hygromycin B. Biological Engineering Progress . 1990
  • 10Huang, D. F,Zhang, J,Zhang, Z. G. et al.Gene transformation of pathogen of cotton wilt. Progress in Natural Science . 1990
Journal of Harbin Institute of Technology(New Series)
2005年 第1期

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