摘要
用１１种限制性内切酶（ＢａｍＨⅠ、ＢｇｌⅠ、ＢｇｌⅡ、ＢｃｏＲⅠ、ＨｐａⅠ、ＫｐｎⅠ、ＰｓｔⅠ、ＳａｃⅠ、ＳａｌⅠ、ＸｈｏⅠ、ＸｂａⅠ）分析鳙鱼的线粒体ＤＮＡ（ｍｉｔｏｃｈｏｎｄｒｉａｌＤＮＡ，简称ｍｔＤＮＡ），构建了全部１１种酶２２个切点的物理图谱．鳙鱼线粒体ＤＮＡ的分子大小约为１６．４４ｋｂ．酶切位点的分布是非随机的．
The mtDNA of Aristichthys has been isolated and purified for restriction enzyme analysis. Its length is 16.44 kb,and its molecular weight is 10.17×108 dation. The mtDNA has been analyzed by 11 kinds of restriction endonucleases which all recognized 6 basepairs. The restriction mapof the total 22 cut Sites have been constructed according to the data of molecular weight of generated fragmentS by complete digestion with single and double enzymes,sometimes also with partial digestionof single enzyme. The distribution of endonuclease sites is not even on the mtDNA of this kind of fish.
出处
《武汉大学学报（自然科学版）》
CSCD
1994年第1期121-125,共5页
Journal of Wuhan University(Natural Science Edition)
关键词
鳙鱼
线粒体
DNA
限制性内切酶
Aristichthys nobilis, mtDNA,restriction enzyme, Physical map
