摘要
目的 探讨慢性移植肾肾病大鼠模型肾组织中单核细胞趋化因子1(MCP 1)和RANTES的表达及意义。方法 以F344大鼠为供者、Lewis大鼠为受者建立慢性移植肾肾病(CAN)大鼠模型,以切除单侧肾脏的F344大鼠和Lewis大鼠为对照。术后12周,测定各组大鼠的肾功能以及肾组织中MCP 1和RANTES的表达,并观察同期肾组织学改变。结果 与单纯切肾的F344大鼠比较,CAN大鼠的血清肌酐水平明显升高(P<0.05),每100 g体重肌酐清除率明显降低(P<0.01);CAN大鼠肾组织的Banff评分明显高于单纯切肾的F344大鼠和Lewis大鼠(P<0.01); CAN大鼠肾组织中MCP 1 和RANTES的表达明显高于单纯切肾的F344大鼠(P<0.05,P<0.01);CAN大鼠移植肾组织学改变与肾组织中MCP 1和RANTES的表达呈正相关关系(r=0.5543,P<0.05;r=0.6259,P<0.05)。结论肾组织中MCP 1和RANTES的表达升高可能与慢性移植肾肾病的发生有关。
Objective To investigate the expression of monocyte chemoattractant protein 1 (MCP-1) and RANTES and their significance in the pathogenesis of chronic allograft nephropathy.Methods Uninephrectomized F344 rats and LEW rats were used as control animals. Left renal transplantation LEW rats were used as experimental animals. Twelve weeks after transplantation, the renal function and the histological disorders of chronic allograft nephropathy were studied. The expression of MCP-1 and RANTES in the allografts was detected by immunohistochemistry.Results Compared with F344 control groups, the serum creatitine level ( 96.200 ± 36.405 ) μmol/L in the experimental groups was increased ( P < 0.05 ), the creatitine clearance ( 3.00 ± 1.62 ) μl·s -1 ·100g -1 decreased ( P < 0.05 ). Compared with F344 and LEW controls, the histological disorders of the experimental group (Banff Sum score 4.550 ± 1.863 ) were more significant ( P < 0.05 ), the expression of MCP-1 and RANTES were significantly up-regulated in the experimental group ( P < 0.01 ). The degree of histological change of allografts was significantly correlated with MCP-1 and RANTES expression respectively ( r = 0.5540 , P < 0.05 ; r = 0.6259 , P < 0.05 ).Conclusion The up-regulated expression of MCP-1 and RANTES in transplant kidney tissue may play a role in the pathogenesis of chronic allograft nephropathy .
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第3期157-159,共3页
Chinese Journal of Organ Transplantation
