摘要
建立一种简单、快速的反相高效液相色谱(RP-HPLC)法测定穿心莲、路边青配伍药液中脱水穿心莲内酯在小鼠血浆中的浓度.使用C18反相柱(4.0mm×250mm,5μm),流动相为甲醇-乙腈-水(13∶17∶70),流速:1.0mL·min-1,检测波长:252nm.血药浓度在0.102～20.305mg·L-1(r=0.9998)时线性关系良好;低、中、高浓度下的回收率、重现性均符合方法学要求.该方法能有效地监测小鼠血浆中脱水穿心莲内酯的浓度变化.
To develop a simple and rapid reversed-phase high performance liquid chromatographic method for the determination of dehydroandrographolide in Andrographis and Clerodendron compatible preparation in mice plasma.The C_(18) reversed-phase column(4.0mm×250mm,5μm)was used as analytical column;The mobile phase consisted of methanol-acetonitrile-water (13∶17∶70) at a flow rate of 1.0mL·min^(-1);The detection wavelength was 252 nm. The calibration curve in plasma was linear well over the range of 0.102～20.305mg·L^(-1)(r=(0.9998));The recovery and reproducibility of low、middle and high concentration are accord with methodological request. The method is proved suitable for monitoring the change of concentration of dehydroandrographolide in mice plasma.
出处
《湖北大学学报（自然科学版）》
CAS
北大核心
2005年第1期74-77,共4页
Journal of Hubei University：Natural Science
基金
湖北大学中药生物技术省重点实验室开放基金(040104)资助项目
湖北省青年杰出人才科学基金(2002AC004)资助项目
关键词
脱水穿心莲内酯
血浆
路边青
小鼠
配伍
RP—HPLC
药液
浓度
线性关系
使用
RP-HPLC
Andrographis and Clerodendron compatible preparation
dehydroandrographolide
determination of plasma concentration
