摘要
利用改进的差异显示PCR(DDRT-PCR)技术从水稻细胞中分离与缺铁胁迫相关的新基因。在分离到的8个差异片段中,通过Northern杂交验证,其中5个为阳性。在这5个阳性片段中有3个是受缺铁胁迫抑制,有2个受缺铁胁迫诱导表达。对这些阳性片段的序列分析表明有3个(IDR2,IDR3,IDR8)为新基因。其中IDR2、IDR8是2个未知基因,IDR3通过GenBank搜索可以找到一些与它同源的已知基因片段。
An improved differential display reverse transcript PCR(DDRT-PCR) approach was used to identify novel genes treated by iron-deficiency from rice cells.Total eight differential fragments(IDR1-8) were identified.Further northern blotting indicated that five of them were positive;three of those positive fragments were suppressed in iron-deficiency condition,while two of them were induced under iron-deficiency.Sequence analysis revealed that three of them (IDR2,IDR3,IDR8) represent novel genes.Two of them (IDR2,IDR8) were unknown genes,while the IDR3 was homogenous to some known genes by blasting approach.
出处
《武汉植物学研究》
CSCD
北大核心
2005年第1期7-10,共4页
Journal of Wuhan Botanical Research
基金
国家973计划项目(2001CB1008-05)资助
国家863计划项目(2002AA207001)资助。
