摘要
采用He- Ne激光对茯苓菌F9进行了 2次照射诱变处理 ,选育到 2株生长速率和产量均有较大提高的二次激光诱变株F2 . 10及F2 . 9。它们在PDA平板上培养 4d ,生长速率分别比原始菌株F9提高了 91 .1%和86 .8%。摇瓶发酵周期缩短了 2d左右 ,摇瓶发酵 5d后 ,它们的生物量最高 ,分别达到 39. 1g/ 10 .0mL和 37 .7g/ 10. 0mL ,比原始菌株F9培养 7d的最高生物量 2 .4 g/ 10. 0mL分别提高了 6 2 . 9%和 5 7 .1%。经传代培养分析 ,诱变株的产量性状稳定。表明激光诱变是获得高产茯苓菌的有效途径。
Two Poria cocos mutants F2.10, F2.9 whose mycelia growth speed and yield were much higher than that of the parental strain F9,were obtained by twice irradiation using He-Ne laser. Their mycelia growth rate was increased by 91.1% and 86.8% respectively when cultured for 4 days on PDA plates.Their biomass could reached 39.1g/100 mL and 37.7g/100 mL after 5-day fermentation, which was increased by 62.9% and 57.1% respectively compared with 24g/100 mL of the original strain F9. The mutants have good hereditary stability subculturing after several times.Our results showed that laser-inducing was an effective ways for screening excellent Poria cocos strains.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2005年第2期51-54,共4页
Food and Fermentation Industries
