摘要
初步探讨从中国毛虾(Aceteschinensis)经碱性及中性蛋白酶降解获得的多肽(AP、NP)的降压作用及相关机制。采用Cushman-Cheung方法测定酶解多肽体外对血管紧张素酶(ACE)活性的影响;采用两肾一夹型建立肾血管性高血压大鼠模型,分别长期预防给药及急性治疗给药;应用GY-6088型多道生理参数分析记录仪和张力传感器测定、记录血压和呼吸;采用均相竞争法测定大鼠血浆中血管紧张素Ⅱ(AngⅡ)的含量。结果表明,2种酶解多肽体外均能明显抑制ACE的活性,AP和NP的半数抑制浓度(IC50)分别为15.61mg/L和16.95mg/L;无论是长期预防给药还是急性治疗给药,二者均能显著地降低肾血管性高血压大鼠的动脉血压;经口服预防给药能明显降低高血压大鼠血浆中的AngⅡ水平;给药大鼠的呼吸频率随血压的变化呈正相关变化;碱性酶解多肽的降压作用及抑制ACE生成的作用均强于中性酶解多肽,其中,200μg/g的AP降压效果最好,与14μg/g的卡托普利相当。中国毛虾酶解多肽无论是长期预防给药还是急性治疗给药对肾性高血压大鼠均有显著的降压作用,抑制AngⅡ的生成可能是其降压机制之一。
To investigate the antihypertensive effects of polypeptides prepared from Acetes chinensis on ren- ovascular hypertensive rats (RHR) and related mechanisms, polypeptides were prepared from Acetes chinensis with alkaline and neutral proteases, and the effects of these two polypeptides on the activity of ACE were measured by Cushman-Cheung assay in vitro. RHR models were made with two-kidney one clip assay, and antihypertensive effects were studied in RHR pretreated with polypeptides for long course or by acute venous injection. Arterial blood pressure, respiration and plasma angiorensin Ⅱ(Ang Ⅱ) were measured respectively. The twopolypeptides markedly inhibited the activity of ACE in vitro with IC50 of AP (15.61mg/L) and NP (16.95 mg/L). They remarkably reduced the arterial blood pressure in the two cases referred above. Plasma Ang Ⅱ of RHR greatly decreased after pretreated with the polypeptides. Respiration of RHR had the same variation trend as blood pressure. Moreover, AP showed stronger antihypertensive and Ang Ⅱ -reducing effects than that of NP. The effects of 200 μ g/g AP were similar to that of 14 μg/g captopril. Polypeptides from Acetes chinensis showed strong antihypertensive effects in RHR. One possible mechanisms underlying the antihypertensive effects may be associated with its actions on decreasing the production of Ang Ⅱ.
出处
《海洋科学》
CAS
CSCD
北大核心
2005年第3期20-24,共5页
Marine Sciences
