摘要
目的 观察同型半胱氨酸 (Hcy) 对人血管平滑肌细胞 (VSMCs) 凋亡以及bax、bcl-2表达的影响。方法 采用组织贴块法体外培养人VSMCs; 采用流式细胞仪检测细胞凋亡率; 逆转录 -聚合酶链反应法检测Bax、bcl-2mRNA的表达。结果 体外培养的人VSMCs在终浓度为 0 (对照组)、100、200、500、1 000μmol/LHcy的培养液中孵育 24h后的细胞凋亡率分别为 ( 2. 68±0. 23 )%、 ( 2. 79±0 .12 )%、( 8. 45±2. 41 )%、 ( 13. 37±4 .71 )%、(23. 75±5 .56)%, 表明Hcy诱导人VSMCs细胞凋亡呈浓度依赖性; baxmRNA相对表达量分别为 0 .86±0. 01、0 .92±0 .03、1. 51±0 .02、1. 82±0 .03、1 .91±0 .02, bcl-2mRNA相对表达量分别为 1. 38±0 .04、1. 32±0 .03、1. 28±0 .03、1 .21±0. 02、1 .09±0. 02, 表明Hcy呈浓度依赖性诱导人VSMCsbax基因表达显著上调, bcl-2基因表达轻度下调, bax/bcl-2比值升高。结论 Hcy诱导人VSMCs凋亡可能是通过上调bax基因表达来实现的, 诱导人VSMCs凋亡可能是Hcy致动脉粥样硬化作用的机制之一。
Objective To investigate the effects of Homocysteine (Hcy) on the apoptosis of human vascular smooth muscle cells (HVSMCs) cultured in vitro and expression of bax and bcl-2 gene.Methods HVSMCs were cultured in vitro,the rate of apoptosis of HVSMCs was detected by the way of flow cytometry;the expression of bax and bcl-2 mRNA of HVSMCs was detected by the way of reverse-transcriptase polymerase chain reaction (RT-PCR).Results The rate of apoptosis of HVSMCs incubated with 0,100,200,500,1 000μmol/L Hcy for 24 h was (2.68±0.23)%,(2.79±0.12)%,(8.45±2.41)%,(13.37±4.71)%,(23.75±5.56)%,respectively,revealing that Hcy could induce the apoptosis of HVSMCs in a concentration-dependent manner;the expression of bax mRNA was 0.86±0.01,0.92±0.03,1.51±0.02,1.82±0.03,1.91±0.02,respectively,and the expression of bcl-2 mRNA was 1.38±0.04,1.32±0.03,1.28±0.03,1.21±0.02,1.09±0.02,respectively,revealing that Hcy could up-regulate bax gene of VSMCs significantly and down-regulate bcl-2 gene relatively weakly,resulting in an enhancement of the ratio of bax/bcl-2.Conclusion Hcy can induce the apoptosis of HVSMCs by up-regulating the expression of bax gene,which may be one of the mechanisms involved in atherosclerotic effects of Hcy.
出处
《中国全科医学》
CAS
CSCD
2005年第6期446-449,共4页
Chinese General Practice
