摘要
目的:复习文献对14例脾脏原发性恶性淋巴瘤(PrimaryLymphomaofSpleen,PLS)进行免疫组化的研究,并检测其p53的表达情况。方法:对瘤组织采用常规石蜡包埋、切片、HE染色及SP两步法免疫组化染色,光镜观察。结果:免疫组化染色14例瘤细胞均表达CD45阳性,13例B细胞性均表达CD20阳性,其中B-小淋巴细胞淋巴瘤(3/14例),可表达CD43、CD79α和bcl2阳性;脾边缘区B细胞淋巴瘤(4/14例),可表达CD79α、IgM、ALK阳性,IgD弱阳性;淋巴浆细胞样淋巴瘤(2/14例),表达CD43、CD79α、bcl2、IgM阳性,IgD阴性;弥漫性大B细胞淋巴瘤(4/14例),表达ALK阳性,CD30、CD3弱阳性。1例周围T细胞淋巴瘤,无其他特征型(1/14例),表达CD45Ro、CD3阳性,CD30弱阳性。14例PLS中6例(42.9%)表达p53阳性,而21例慢性增生性脾炎对照组中仅1例(5%)表达p53弱阳性,两组间有显著差异(P<0.05)。结论:PLS较罕见,免疫组化染色对其诊断,鉴别诊断及分型具有重要的意义。PLS多为B细胞型,普遍认为与脾脏白髓中B细胞恶性变有关。PLS中可有p53异常蛋白表达,其预后较脾脏继发性恶性淋巴瘤及其它恶性肿瘤为好。
Objective: To review the literature concerned and to investigate the characteristics of clinico-pathology, the results of immunohistochemistry stain for primary lymphoma of spleen (PLS) in 14 cases. Methods: The routine paraffin slides were cut, stained with H. E and immunochemically stained with ABC method, and then, they were observed under optical microscopy. Results: On the immunochemically stained slides, the tumor cells of all the 14 cases expressed LCA positive, among them 13 cases exepressed CD20, 1 cases CD45Ro positive repectively. On the immunochemical stained slides, the tumor cells of 6 (42. 9% ) expressed p53 positive. In 21 case of control group, only 1 case (5% ) expressed p53 weak positive. There was significant differnce beteen the two groups (P <0. 05). Conclusion: The PLB was relatively rare. It should be differentiated from other primary or metastatic small cell malignant tumor of spleen. Immunohistochemistry stain play a crucial role in diagnosis, differential diagnosis, subdivision of the types of PLS, and identifing the origination of the lymphoma cells. Most of PLS was B cell Lymphoma and its o-rigination might be related to malignant change of B lymphocytes in bone marrow. Among the PLS, the most cases of large cell lymphomas formerly called reticular sarcomas were now considerated to be originated from lymphocytes. Although PLS originated from histiocyte was rare, our study indicated that this type of lymphoma was really existent.
出处
《江苏大学学报（医学版）》
CAS
2005年第1期62-64,66,共4页
Journal of Jiangsu University：Medicine Edition
基金
江苏省卫生厅科技资助项目(H200220)国家教育部留学回国人员科研启动基金资助项目(1997-436)
关键词
脾脏原发性肿瘤
淋巴瘤
临床病理
免疫组化
基因
P53
Primary splenic neoplasms
Lymphoma
Clinicopathology
Immunohistiochemistry
Gene
p53
