摘要
以禽类皮肤组织为原料 ,经过酸液预处理 ,热水抽提法提取胶原蛋白 ,并通过酸法水解、有机溶剂沉淀分离、干燥等工艺过程 ,制备胶原水解多肽。所得成品的多肽平均分子质量 7.7kDa,收率为原料湿重的 3 .5 %～7.0 % ,水溶性良好 ,含氮量达 1 6% ,氨基酸含量达 84% (羟脯氨酸含量未计 ) ,重金属含量小于 1 0mg/kg。文中还对禽源性皮胶原蛋白的提取原理和影响水解过程的因素 ,进行了分析。
Epidermal tissue of fowl-based source was soaked and pretreated by acid solution and used to produce collagen gelatin. Collagen polypeptides were then prepared by hydrolyzing the collagen gelatin with acid, as well as by separation and drying processes. The obtained collagen polypeptides product has good water-solubility and with the averaged molecule weight of 7.7 kDa, the nitrogen content of 16%, the total amino acids content of more than 84% (without accounting the hydroxyproline content), the content of heavy metals less than 10 mg/kg and the dried collagen polypeptides yield of 3.5% to 7.0% compared with the crude epidermal tissue. The extracting principles and the influence factors on the hydrolyzing process were also discussed.
出处
《中国皮革》
CAS
北大核心
2005年第3期38-40,共3页
China Leather
关键词
皮胶原蛋白
多肽
禽类皮肤组织
提取
水解
skin collagen protein polypeptide epidermal tissue of fowl extraction hydrolysis
